Recent progress in quantitative glycoproteomics
Recent progress in quantitative glycoproteomics
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定量糖蛋白组学最新进展
DOI：
10.1007/s10719-012-9398-x
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发表时间：
2012-08-01
影响因子：
3
通讯作者：
Lu, Haojie
中科院分区：
文献类型：
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作者：
Zhang, Ying;Yin, Hongrui;Lu, Haojie
蛋白质糖基化被认为是一种主要的翻译后修饰，对蛋白质的折叠、构象、分布、稳定性和活性产生重大影响。糖蛋白丰度、糖基化程度和聚糖结构的变化与多种疾病有关。因此，糖蛋白组学的定量研究已成为一个新的热门研究课题，并迅速成为生物标志物发现的重要技术。基于质谱的蛋白质定量技术为系统和定量地评估不同样品中蛋白质谱的定量差异提供了强有力的工具。结合各种糖蛋白/糖肽富集策略和其他糖蛋白分析方法，这些技术在定量糖蛋白组学中的应用得到了进一步的发展。由于生物样品的巨大复杂性、糖蛋白的内在特性以及缺乏通用的定量技术，对复杂生物样品中的糖蛋白组进行全面的定量分析仍然具有挑战性。本文综述了近年来糖蛋白组定量研究的最新进展，特别是针对两种最常见的糖基化类型（n -链和o -链糖蛋白组）的研究进展。还讨论了各种方法的优缺点。
Protein glycosylation is acknowledged as one of the major posttranslational modifications that elicit significant effects on protein folding, conformation, distribution, stability, and activity. The changes in glycoprotein abundance, glycosylation degree, and glycan structure are associated with a variety of diseases. Therefore, the quantitative study of glycoproteomics has become a new and popular research topic, and is quickly emerging as an important technique for biomarker discovery. Mass spectrometry-based protein quantification technologies provide a powerful tool for the systematic and quantitative assessment of the quantitative differences in the protein profiles of different samples. Combined with various glycoprotein/glycopeptide enrichment strategies and other glycoprotein analysis methods, these techniques have been further developed for application in quantitative glycoproteomics. A comprehensive quantitative analysis of the glycoproteome in a complex biological sample remains challenging because of the enormous complexity of biological samples, intrinsic characteristics of glycoproteins, and lack of universal quantitative technology. In this review, recently developed technologies in quantitative glycoproteome, especially those focused on two of the most common types of glycosylation (N-linked and O-linked glycoproteome), were summarized. The strengths and weaknesses of the various approaches were also discussed.