Immunohistochemical co-localization of transient receptor potential vanilloid (TRPV)1 and sensory neuropeptides in the guinea-pig respiratory system
Immunohistochemical co-localization of transient receptor potential vanilloid (TRPV)1 and sensory neuropeptides in the guinea-pig respiratory system
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DOI：
10.1016/j.neuroscience.2006.04.073
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发表时间：
2006-01-01
期刊：
影响因子：
3.3
通讯作者：
Priestley, J. V.
中科院分区：
文献类型：
--
作者：
Watanabe, N.;Horie, S.;Priestley, J. V.
肺内的电生理学研究已经证明存在由A δ和C-纤维组成的异源传入纤维组，并且皮肤内的体感神经的研究揭示了感觉神经肽和瞬时受体电位香草酸（TRPV）1阳性神经的复杂分布模式。然而，肺内这些不同神经亚群的解剖位置尚未得到广泛研究。在本研究中，我们已经证明TRPV 1轴突仅代表豚鼠气管上皮内PGP9.5染色神经总数的一小部分，并且只有一半数量的TRPV 1轴突对P物质呈免疫阳性。在豚鼠肺内气道内发现的大多数TRPV 1阳性神经元与感觉神经肽P物质和降钙素基因共定位，上皮内和上皮下、血管周围、气道平滑肌和肺泡内的TRPV 1相关肽，表明整个气道中TRPV 1阳性轴突的异质性。然而，在气管的平滑肌层中，有证据表明含有P物质和降钙素基因相关肽的神经未被TRPV 1染色。我们还证明了在长期用辣椒素治疗的动物中，气管和肺内气道中TRVP 1阳性轴突的完全丧失以及辣椒素诱导的支气管收缩的相关丧失。然而，一些神经肽免疫反应性轴突仍然在辣椒素处理的动物的平滑肌层中，这可能代表了一小部分不与TRPV 1共定位的神经肽纤维。我们已经提供了TRPV 1阳性神经纤维异质性的证据，包括在气道的各个区域中缺乏与神经肽共定位的纤维，以及在豚鼠中存在非TRPV 1阳性的含有神经肽的纤维。(c)2006年IBRO。由Elsevier Ltd.出版。保留所有权利。
Electrophysiological studies within the lung have documented the presence of heterogenous groups of afferent fibers composed of A delta and C-fibers and studies of somatosensory nerves within the skin reveal a complex pattern of distribution of sensory neuropeptides and transient receptor potential vanilloid (TRPV)1 positive nerves. However, the anatomical location of these different subpopulations of nerves within the lung has not been extensively studied. In the present study we have demonstrated that TRPV1 axons represented only a small proportion of the total number of PGP9.5 staining nerves within guinea-pig tracheal epithelium and only half the number of TRPV1 axons was immunopositive for substance P. In contrast, most TRPV1 positive neurones found within guinea-pig intrapulmonary airways were found to co-localize with sensory neuropeptides substance P and calcitonin gene-related peptide within and beneath the epithelium, around blood vessels, within airway smooth muscle and alveoli, indicative of heterogeneity of TRPV1 positive axons throughout the airways. However, in the smooth muscle layer of the trachea there was evidence of substance P and calcitonin gene-related peptide containing nerves that did not stain for TRPV1. We also demonstrated a complete loss of TRVP1 positive axons in the trachea and intrapulmonary airways and associated loss of bronchoconstriction induced by capsaicin, in animals chronically treated with capsaicin. However, some neuropeptide immunoreactive axons remained in the smooth muscle layer of capsaicin-treated animals which could represent the small subset of neuropeptide containing fibers which do not co-localize with TRPV1. We have provided evidence of heterogeneity of TRPV1 positive nerve fibers, including fibers characterized by lack of co-localization with neuropeptides in various regions of the airways and the existence of neuropeptide containing fibers that were not TRPV1 positive in guinea-pigs. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.