Effect of carnitine analogs on carnitine acetyltransferase.
Effect of carnitine analogs on carnitine acetyltransferase.
复制标题
肉碱类似物对肉碱乙酰转移酶的影响。
DOI：
10.1002/jps.2600690223
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发表时间：
1980
影响因子：
3.8
通讯作者：
J. L. Bobbitt
中科院分区：
文献类型：
--
作者：
M. R. Boots;M. Wolfe;S. Boots;J. L. Bobbitt
研究了氮上不同取代基的肉碱类似物对大鼠精子和鸽胸肉碱乙酰转移酶的影响。在精子制备中有其他酶竞争乙酰辅酶A的情况下，采用放射测定法测量乙酰肉碱的形成。类似物对酶的明显抑制作用可以解释为类似物作为替代底物具有较高的Km和较低的Vmax值。类似物对整个精子没有影响。
Carnitine analogs with various substituents on the nitrogen were tested for their effect on carnitine acetyltransferase from rat sperm and pigeon breast. A radiometric assay was used to measure the formation of acetylcarnitine in the presence of other enzymes that competed for acetyl coenzyme A in the sperm preparation. The apparent enzyme inhibition caused by the analogs was explained by the analogs serving as alternative substrates with higher Km and lower Vmax values. The analogs had no effect on whole sperm.