Conserved interaction of the papillomavirus E2 transcriptional activator proteins with human and yeast TFIIB proteins
Conserved interaction of the papillomavirus E2 transcriptional activator proteins with human and yeast TFIIB proteins
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DOI：
10.1128/jvi.71.10.8041-8047.1997
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发表时间：
1997-10-01
影响因子：
5.4
通讯作者：
Howley, PM
中科院分区：
文献类型：
--
作者：
Benson, JD;Lawande, R;Howley, PM
乳头瘤病毒早期基因表达受病毒基因编码的E2蛋白调控。由牛乳头瘤病毒1型（BPV-1）编码的最具特征的E2蛋白已被证明与基础转录因子IIB（TFIIB）和TATA结合蛋白基础转录因子（N. M. Rank和P. F，Lambert，J，Virol. 69：6323-6333，1995）。我们证明，由人PV 16型基因编码的有效E2转录激活蛋白也与TFIIB在体外相互作用。此外，VP 16和BPV-1 E2所需的人TFIIB（hTFIIB）内结构域的直接比较表明，这些酸性活化剂以定性相似的方式与hTFIIB相互作用。我们的作图实验鉴定了BPV-1 E2的氨基末端活化结构域内的hTFIIB相互作用结构域。最后，我们证明了在体外酿酒酵母TFIIB和EPV-1 E2之间的相互作用，观察是一致的重要性BPV-1 E2反式激活E2-TFIIB相互作用在这两个系统。
Papillomavirus early gene expression is regulated by the virus gene encoded E2 proteins. The best-characterized E2 protein, encoded by bovine papillomavirus type 1 (BPV-1), has been shown to interact with basal transcription factor IIB (TFIIB) and the TATA binding protein basal transcription factor (N. M. Rank and P. F, Lambert, J, Virol. 69:6323-6333, 1995). We demonstrate that the potent E2 transcriptional activator protein encoded by a gene of human PV type 16 also interacts with TFIIB in vitro. Moreover, a direct comparison of domains within human TFIIB (hTFIIB) required for VP16 and BPV-1 E2 indicates that these acidic activators interact with hTFIIB in a qualitatively similar manner. Our mapping experiments identify hTFIIB interaction domains within the amino-terminal activation domain of BPV-1 E2. Finally, we demonstrate in vitro interaction between Saccharomyces cerevisiae TFIIB and EPV-1 E2, an observation that is consistent with the importance of the E2-TFIIB interaction for BPV-1 E2 transactivation in both systems.