Morin inhibits colorectal tumor growth through inhibition of NF-κB signaling pathway
Morin inhibits colorectal tumor growth through inhibition of NF-κB signaling pathway
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DOI：
10.1080/08923973.2019.1688344
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发表时间：
2019-11-12
影响因子：
3.3
通讯作者：
Zhang, Li
中科院分区：
文献类型：
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作者：
Chen, Rui;Zhang, Li
背景：探讨桑色素对人结直肠癌的作用及其机制。方法：采用四甲基偶氮唑盐比色法和集落形成实验检测桑色素对体外培养的人结肠癌细胞增殖的影响。采用异种移植瘤模型，观察桑色素对结直肠癌生长的影响。用酶联免疫吸附试验检测炎性细胞因子的产生。采用定量逆转录聚合酶链式反应(qRT-PCR)和免疫印迹法检测桑色素对HCT-116细胞p65-NF-kappaB表达和活化的影响。采用定量RT-PCR、免疫印迹和免疫荧光染色等方法检测C/EBPβ诱导的p65-NF-kappa B表达上调对桑色素抑制HCT-116细胞生长的影响。结果：桑色素在体外对人结直肠癌细胞增殖有抑制作用，在体内对结直肠癌生长有抑制作用。桑色素可抑制肿瘤坏死因子-α诱导的HTC-116细胞p65-NF-kappaB的表达和活化，以及IL-6和IL-8的产生。桑色素的抑制作用可被C/EBPβ诱导的p65-NF-kappaB上调所阻断。桑色素在体内外对人结直肠癌均有抑制作用。结论：桑色素对人大肠癌细胞的抗肿瘤作用可能部分是通过抑制核因子-kappaB信号转导途径实现的。
Background: To explore the effects and underlying mechanisms of Morin in human colorectal cancer. Methods: Effects of Morin on proliferation of human colorectal cells in vitro were detected by MTT and colony formation assay. Effects of Morin on growth of colorectal tumors in vivo were examined using the xenograft tumor model. Production of inflammatory cytokines was evaluated by ELISA assay. Effects of Morin on p65-NF kappa B expression and activation in HCT-116 cells were determined by qRT-PCR and western blot. Effects of C/EBP beta-induced upregulation of p65-NF kappa B on the inhibitory effects of Morin in HCT-116 cells were evaluated by qRT-PCR, western blot and immunofluorescent staining analysis. Results: Morin inhibited cell proliferation of human colorectal cells in vitro and growth of colorectal tumors in vivo. Morin treatment suppressed TNF-alpha-induced p65-NF kappa B expression and activation as well as production of IL-6 and IL-8 in HTC-116 cells. The inhibitory effects of Morin were abrogated by C/EBP beta-induced upregulation of p65-NF kappa B. Morin had inhibitory effects on human colorectal cancer both in vitro and in vivo. Conclusion: The antitumor functions of Morin in human colorectal cancer cells might be partially due to inhibition of NF-kappa B signaling.