The composition and transmission of microbiome in hard tick, Ixodes persulcatus, during blood meal
The composition and transmission of microbiome in hard tick, Ixodes persulcatus, during blood meal
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DOI：
10.1016/j.ttbdis.2014.07.009
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发表时间：
2014-01-01
影响因子：
3.2
通讯作者：
Xu, Hai-Jun
中科院分区：
文献类型：
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作者：
Zhang, Xue-Chao;Yang, Zhang-Nv;Xu, Hai-Jun
全沟硬蜱是我国东北地区的优势蜱种，是蜱传疾病的主要传播媒介。通过16S rRNA Illumina测序，我们研究了I. persulcatus和评估之前和之后的血液喂养的微生物组的变化。长时间的血餐极大地改变了微生物组的组成，但没有影响细菌的多样性。总体而言，373和289细菌属分别分配给未进食和进食蜱。为了研究在血餐期间可能传播给脊椎动物宿主的微生物，我们检查了蜱叮咬后大鼠血液中的微生物组。我们的数据显示，237个细菌属被怀疑是脊椎动物的病原体，因为它们通常在未进食的蜱、进食的蜱和蜱叮咬后的大鼠血液样品中检测到。此外，对伯氏疏螺旋体s.l.的流行率调查，查菲埃立克体、嗜吞噬细胞无形体和鼠疫耶尔森氏菌。我们找到了那个B。garinii和B. afzelii是莱姆病螺旋体的优势基因种。全沟蜱这是第一次报道这种蜱种和通过蜱叮咬传播的大鼠血液中的微生物组成。这些数据可能最终有助于识别由全沟乳杆菌蜱传播的新型病原体。(C)2014 Elsevier GmbH. All rights reserved.
The tick Ixodes persulcatus is the predominant tick species in Northeastern China, and it is a major vector in transmission of tick-borne diseases. By 16S rRNA Illumina sequencing, we investigated the microbiome of I. persulcatus and assessed the variation of the microbiome before and after blood feeding. The prolonged blood meal dramatically altered the composition of the microbiome but did not influence the bacterial diversity. Overall, 373 and 289 bacterial genera were assigned to unfed and fed ticks, respectively. To investigate microbes that were potentially transmitted to vertebrate hosts during a blood meal, we examined the microbiome in rat blood after tick bites. Our data showed that 237 bacterial genera were suspected to be pathogens of vertebrates because they were commonly detected in unfed ticks, fed ticks, and rat blood samples after tick bites. Additionally, the prevalence survey on Borrelia burgdorferi s.l., Ehrlichia chaffeensis, Anaplasma phagocytophilum and Yersinia pestis was performed. We found that B. garinii and B. afzelii are the predominant genospecies of the Lyme disease spirochete in I. persulcatus ticks. This is the first time that the microbial composition in this tick species and in rat blood transmitted via tick bites has been reported. These data may ultimately assist in identification of novel pathogens transmitted by L persulcatus ticks. (C) 2014 Elsevier GmbH. All rights reserved.