Antioxidant activity of melatonin in Chinese hamster ovarian cells: changes in cellular proliferation and differentiation.
Antioxidant activity of melatonin in Chinese hamster ovarian cells: changes in cellular proliferation and differentiation.
复制标题
中国仓鼠卵巢细胞褪黑素的抗氧化活性：细胞增殖和分化的变化。
DOI：
10.1016/s0006-291x(03)00230-4
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发表时间：
2003
影响因子：
3.1
通讯作者：
Reiter,RusselJ
中科院分区：
文献类型：
--
作者：
Sainz,RosaM;Mayo,JuanC;Tan,Dun-Xian;Lopez-Burillo,Silvia;Natarajan,Mohan;Reiter,RusselJ
褪黑激素是一种内源性分子，具有清除自由基和抗氧化特性。在这里，我们研究了褪黑激素和其他抗氧化剂对转化的中国仓鼠卵巢细胞的抗增殖作用。褪黑激素以剂量和时间依赖性方式减少细胞增殖。天然抗氧化剂出现在可食用植物中，包括白藜芦醇和维生素E，模仿褪黑激素的作用。流式细胞仪分析显示，褪黑激素治疗减少了S期细胞的数量，并增加了G 0/G1和G2/M间隙的细胞。此外，褪黑激素，以及trolox，通过诱导细胞成为梭形和成纤维细胞样引起了明显的形态学变化。它的作用是一种可逆的现象，当褪黑激素从培养基中撤出时消失。谷胱甘肽水平增加后，褪黑激素治疗，但谷胱甘肽合成的药物阻断并没有取消褪黑激素的抗增殖作用。细胞增殖的减少和细胞分化的明显诱导与褪黑激素改变CHO细胞的细胞内氧化还原状态的能力重叠。我们的结论是，细胞的氧化还原状态可能参与抗氧化剂，如褪黑激素和trolox引起的细胞转化。
Melatonin is an endogenously generated molecule with free radical scavenging and antioxidant properties. Here, we studied the antiproliferative role of melatonin and other antioxidants on transformed Chinese hamster ovarian cells. Melatonin reduces cell proliferation in a dose- and time-dependent manner. Natural antioxidants which appear in edible plants including resveratrol and vitamin E mimicked the effect of melatonin. Flow cytometer analysis revealed that melatonin treatment reduces the number of cells in S-phase and increases cells in both G0/G1 and G2/M gaps. In addition, melatonin, as well as trolox, caused a clear morphological change by inducing the cells to become spindle shaped and fibroblast-like. Its effect is a reversible phenomenon that disappeared when melatonin was withdrawn from the culture medium. GSH levels are increased after melatonin treatment but pharmacologically blockade of GSH synthesis did not abolish melatonin’s antiproliferative effect. Reduction of cell proliferation and the apparent induction of cell differentiation overlapped with melatonin’s ability to change the intracellular redox state of CHO cells. We conclude that the cellular redox state may be involved in cellular transformation caused by antioxidants such as melatonin and trolox.