Sampling-dependent up-regulation of gene expression in sequential samples of human airway epithelial cells
Sampling-dependent up-regulation of gene expression in sequential samples of human airway epithelial cells
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DOI：
10.2119/2003-00051.crystal
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发表时间：
2003-09-01
影响因子：
5.7
通讯作者：
Crystal, RG
中科院分区：
文献类型：
--
作者：
Heguy, A;Harvey, BG;Crystal, RG
作为人体气道上皮细胞对慢性吸烟反应的体内基因表达水平研究的一部分，我们已经鉴定了许多基因，这些基因的表达水平因上皮细胞采样程序而以时间依赖性方式发生改变。使用纤维支气管镜检查和气道上皮刷拭从单个个体中获得独立样本，首先从右肺取样，然后从左肺取样。我们观察到，与右肺样本（第1个采样区域）相比，左肺样本（第2个采样区域）中编码参与转录、信号转导、细胞周期/生长和凋亡的蛋白质的早期应答基因的特定子集显著上调。这种反应是由于采样过程的时间性质，而不是右肺和左肺气道上皮细胞之间固有的基因表达差异。当采样顺序颠倒时，首先采样左气道上皮，从右气道上皮获得的样品中相同的基因子集上调。这些基因的时间依赖性上调可能是对手术压力和/或所用麻醉的反应。基因表达的采样依赖性不确定性可能是与用于获得生物样品的程序相关的一般现象，特别是在采样程序依赖于确保舒适和安全的人类中。
As part of a study of in vivo gene expression levels in the human airway epithelium in response to chronic cigarette smoking, we have identified a number of genes whose expression levels are altered in a time-dependent fashion resulting from the procedure used to sample epithelial cells. Fiberoptic bronchoscopy and airway epithelium brushing were used to obtain independent samples from a single individual, 1st from the right lung, followed by sampling of the left lung. We observed that a specific subset of early response genes encoding proteins involved in transcription, signal transduction, cell cycle/growth, and apoptosis were significantly up-regulated in the left lung samples (the 2nd region to be sampled) compared with the right lung samples (the 1st region to be sampled). This response was due to the temporal nature of the sampling procedure and not to inherent gene expression differences between airway epithelium of the right and left lungs. When the order of sampling was reversed, with the left airway epithelium sampled 1st, the same subset of genes were up-regulated in the samples obtained from the right airway epithelium. The time-dependent up-regulation of these genes was likely in response to the stress of the procedure and/or the anesthesia used. Sampling-dependent uncertainty of gene expression is likely a general phenomenon relevant to the procedures used for obtaining biological samples, particularly in humans where the sampling procedures are dependent on ensuring comfort and safety.