Isolation, cloning, and expression mapping of a gene encoding an antidiuretic hormone and other CAPA-related peptides in the disease vector, Rhodnius prolixus
Isolation, cloning, and expression mapping of a gene encoding an antidiuretic hormone and other CAPA-related peptides in the disease vector, Rhodnius prolixus
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DOI：
10.1210/en.2008-0353
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发表时间：
2008-09-01
期刊：
影响因子：
4.8
通讯作者：
Orchard, Ian
中科院分区：
文献类型：
--
作者：
Paluzzi, Jean-Paul;Russell, William K.;Orchard, Ian
血餐后，Rhodnius prolixus进行快速利尿，以消除多余的水和盐。在排尿初级尿液期间，R。prolixus作为南美锥虫病的媒介，其病原体克氏锥虫通过尿液感染人类宿主。R.催吐是在5-羟色胺和肽能利尿激素的神经激素控制下，因此，利尿激素在恰加斯病的传播中起重要作用。虽然利尿激素可能会降解或排泄，导致利尿终止，但鉴于分泌率高，似乎也应该存在有效的抗利尿因子，并在进食后利尿后防止水和盐的过度损失。尽管R.在这种情况下，没有任何神经肽的基因被克隆出来，显然包括那些控制利尿的基因。本研究利用分子生物学技术结合基质辅助激光解吸电离飞行时间串联质谱技术，测定了R. prolixus，其包括具有抗利尿活性的肽。我们的特点是表达的mRNA编码这些肽在不同的发展阶段，并在五龄幼虫的组织特异性分布进行了研究。该表达定位于中枢神经系统内的许多双侧配对的细胞体。此外，我们的研究结果表明，RhoprCAPA基因的表达也与睾丸，这表明一个新的作用，为这个家庭的肽在生殖。
After a blood meal, Rhodnius prolixus undergoes a rapid diuresis to eliminate excess water and salts. During the voiding of this primary urine, R. prolixus acts as a vector of Chagas' disease, with the causative agent, Trypanosoma cruzi, infecting the human host via the urine. Diuresis in R. prolixus is under the neurohormonal control of serotonin and peptidergic diuretic hormones, and thus, diuretic hormones play an important role in the transmission of Chagas' disease. Although diuretic hormones may be degraded or excreted, resulting in the termination of diuresis, it would also seem appropriate, given the high rates of secretion, that a potent antidiuretic factor could be present and act to prevent excessive loss of water and salts after the postgorging diuresis. Despite the medical importance of R. prolixus, no genes for any neuropeptides have been cloned, including obviously, those that control diuresis. Here, using molecular biology in combination with matrix-assisted laser desorption ionization-time of flight-tandem mass spectrometry, we determined the sequence of the CAPA gene and CAPA-related peptides in R. prolixus, which includes a peptide with anti-diuretic activity. We have characterized the expression of mRNA encoding these peptides in various developmental stage and also examined the tissue-specific distribution in fifth-instars. The expression is localized to numerous bilaterally paired cell bodies within the central nervous system. In addition, our results show that RhoprCAPA gene expression is also associated with the testes, suggesting a novel role for this family of peptides in reproduction.