The evolution of the alcohol dehydrogenase gene family by loss of introns in plants of the genus Leavenworthia (Brassicaceae)
The evolution of the alcohol dehydrogenase gene family by loss of introns in plants of the genus Leavenworthia (Brassicaceae)
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DOI：
10.1093/oxfordjournals.molbev.a025955
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发表时间：
1998-05-01
影响因子：
10.7
通讯作者：
Zhang, L
中科院分区：
文献类型：
--
作者：
Charlesworth, D;Liu, FL;Zhang, L
我们目前的研究结果表明，在植物属Leavenoproteia，在芸苔属，有三个醇脱氢酶位点，不像拟南芥，它只有一个经典的（P类）醇脱氢酶位点的几个物种。根据部分序列分析，Leavenaciia的乙醇脱氢酶基因座与A.拟南芥醇脱氢酶绝大多数序列与A.拟南芥Adh编码序列之间的同源性，以及三种不同的Leavenglaia物种之间的同源性，都是同义的，这表明它们目前都是有功能的（或者在最近的进化史中已经有功能）。这些基因座的内含子数目不同，其中一个基因座（Adh-3）没有内含子。RT-PCR检测所有三个位点的表达。利用单链构象多态性分析确定的变异等位基因的连锁数据表明，这三个Leavenoproteia基因座并不紧密连锁。因此，结果表明，Adh-3位点可能已经出现通过mRNA中间体，但尽管损失的内含子，表达。
We present results showing that several species in the plant genus Leavenworthia, in the Brassica family, have three alcohol dehydrogenase loci, unlike Arabidopsis thaliana, which has only a single classical (class P) alcohol dehydrogenase locus. Based on a portion of the sequence, the alcohol dehydrogenase loci of Leavenworthia show about 92%-93% amino acid sequence identity to that of the A. thaliana alcohol dehydrogenase. The great majority of the sequence differences from the A. thaliana Adh-coding sequence, and also between three different Leavenworthia species, are synonymous, suggesting that all are currently functional (or have been in the recent evolutionary past). The loci differ in the numbers of introns present, with one locus (Adh-3) having no introns present. RT-PCR tests detect expression of all three loci. Linkage data using variant alleles identified by single-strand conformation polymorphism analysis show that the three Leavenworthia loci are not closely linked. The results therefore suggest that the Adh-3 locus may have arisen via an mRNA intermediate but, despite loss of the introns, is expressed.