Compensatory increases in tear volume and mucin levels associated with meibomian gland dysfunction caused by stearoyl-CoA desaturase-1 deficiency.
Compensatory increases in tear volume and mucin levels associated with meibomian gland dysfunction caused by stearoyl-CoA desaturase-1 deficiency.
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DOI：
10.1038/s41598-018-21542-3
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发表时间：
2018-02-20
影响因子：
4.6
通讯作者：
Tsubota K
中科院分区：
文献类型：
--
作者：
Inaba T;Tanaka Y;Tamaki S;Ito T;Ntambi JM;Tsubota K
硬脂酰辅酶a去饱和酶（SCD）家族通过在饱和脂肪酸Δ9位置插入顺式双键催化单不饱和脂肪酸的合成。据报道，这些酶的破坏会导致严重的皮肤干燥表型。由于睑板腺脂质异常与干眼症有关，我们分析了SCD-1基因缺陷小鼠（SCD-1 KO）中影响泪液体积和组成的眼组织，包括泪腺和结膜。先前的组织病理学分析显示睑板腺萎缩和丧失；结合本文报道的结膜中杯状细胞和MUC5AC表达的增加，这些发现表明，在没有脂质分泌作为代偿机制的情况下，泪液体积和分泌的粘蛋白水平增加。SCD1 KO小鼠泪腺脂质代谢基因表达降低。因此，这些结果为研究睑板腺功能障碍提供了新的病理生理机制。此外，缺乏SCD-1导致泪液体积和粘蛋白水平代偿性增加，这与脂质代谢基因表达的变化有关。这些结果可能有助于干眼症治疗策略的新概念。
The stearoyl-CoA desaturase (SCD) family of enzymes catalyzes monounsaturated fatty acid synthesis by inserting a cis double bond at the Δ9 position of saturated fatty acids. Disruption of these enzymes has been reported to induce a severe dry skin phenotype. Since lipid abnormalities in the meibomian glands have been associated with dry eye, we analyzed selected eye tissues contributing to tear volume and composition in genetically SCD-1-deficient mice (SCD-1 KO), including the lacrimal glands and conjunctiva. Previous histopathological analysis had revealed atrophy and loss of meibomian glands; taken together with the increased goblet cell and MUC5AC expression in the conjunctiva reported here, these findings suggest that the tear volume and mucin levels secreted are enhanced in the absence of lipid secretion as a compensatory mechanism. The expression of lipid metabolism genes in lacrimal glands was decreased in SCD1 KO mice. Thus, these results provide new pathophysiological mechanisms to pursue with regard to meibomian gland dysfunction. In addition, lack of SCD-1 causes a compensatory increase in the tear volume and mucin levels associated with changes in expression of lipid metabolism genes. These results may be useful as a new concept for dry eye treatment strategies.
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影响因子：
3.7
作者：
Inaba T;Hisatsune C;Sasaki Y;Ogawa Y;Ebisui E;Ogawa N;Matsui M;Takeuchi T;Mikoshiba K;Tsubota K
通讯作者：
Tsubota K
影响因子：
6.5
作者：
Attie, AD;Krauss, RM;Ntambi, JM
通讯作者：
Ntambi, JM
影响因子：
4.2
作者：
Miyazaki, M;Man, WC;Ntambi, JM
通讯作者：
Ntambi, JM
DOI：
10.1073/pnas.132384699
发表时间：
2002-08-20
影响因子：
11.1
作者：
Ntambi, JM;Miyazaki, M;Attie, AD
通讯作者：
Attie, AD
影响因子：
2
作者：
Creech, JL;Do, LT;Radke, CJ
通讯作者：
Radke, CJ