Diaphorobacter nitroreducens gen nov, sp nov, a poly(3-hydroxybutyrate)-degrading denitrifying bacterium isolated from activated sludge.
Diaphorobacter nitroreducens gen nov, sp nov, a poly(3-hydroxybutyrate)-degrading denitrifying bacterium isolated from activated sludge.
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DOI：
10.2323/jgam.48.299
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发表时间：
2002-12
期刊：
影响因子：
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通讯作者：
S. Khan;A. Hiraishi
中科院分区：
文献类型：
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作者：
S. Khan;A. Hiraishi
从活性污泥中分离出三种能够降解聚（3-羟基丁酸酯）（PHB）和聚（3-羟基丁酸酯-co-3-羟基戊酸酯）（PHBV）的反硝化细菌菌株并进行表征。所有分离株都具有几乎相同的表型特征。它们是具有单极性鞭毛的活动革兰氏阴性杆，在有氧和厌氧反硝化条件下，以简单有机化合物以及 PHB 和 PHBV 作为碳和能源生长良好。然而，所测试的糖均不支持它们的生长。细胞脂肪酸谱显示，C16:1omega7cis 和 C16:0 是羟基脂肪酸的主要成分，3-OH-C10:0 是羟基脂肪酸的唯一成分。泛醌-8 被检测为主要的呼吸醌。基于 16S rDNA 序列的系统发育分析表明，所有分离株均属于丛毛单胞菌科（β-变形菌门的一个主要类群），但与该科任何先前已知的物种均不形成单系群。与我们的菌株最接近的是一种未鉴定的细菌菌株 LW1 (=DSM 13225)（99.9% 相似性），之前报道为 1-氯-4-硝基苯降解细菌。新分离株之间的 DNA-DNA 杂交水平超过 60%，而我们的分离株与菌株 DSM 13225 之间的 DNA-DNA 杂交水平低于 50%。新菌株基因组DNA的G+C含量为64～65mol％。基于这些结果，我们得出结论，降解 PHBV 的反硝化菌株应被归类为新属和新种，我们建议将其命名为硝基还原杆菌。模式菌株是菌株NA10B(＝JCM 11421＝CIP 107294)。我们还建议将菌株 DSM 13225 分类为 Diaphorobacter 的一个基因种。
Three denitrifying strains of bacteria capable of degrading poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) were isolated from activated sludge and characterized. All of the isolates had almost identical phenotypic characteristics. They were motile gram-negative rods with single polar flagella and grew well with simple organic compounds, as well as with PHB and PHBV, as carbon and energy sources under both aerobic and anaerobic denitrifying conditions. However, none of the sugars tested supported their growth. The cellular fatty acid profiles showed the presence of C16:1omega7cis and C16:0 as the major components and of 3-OH-C10:0 as the sole component of hydroxy fatty acids. Ubiquinone-8 was detected as the major respiratory quinone. A 16S rDNA sequence-based phylogenetic analysis showed that all the isolates belonged to the family Comamonadaceae, a major group of beta-Proteobacteria, but formed no monophyletic cluster with any previously known species of this family. The closest relative to our strains was an unidentified bacterium strain LW1 (=DSM 13225) (99.9% similarity), reported previously as a 1-chloro-4-nitrobenzene degrading bacterium. DNA-DNA hybridization levels among the new isolates were more than 60%, whereas those between our isolates and strain DSM 13225 were less than 50%. The G+C content of genomic DNA of the new strains was 64 to 65 mol%. Based on these results, we concluded that the PHBV-degrading denitrifying isolates should be classified as a new genus and a new species, for which we propose the name Diaphorobacter nitroreducens. The type strain is strain NA10B (=JCM 11421=CIP 107294). We also propose to classify strain DSM 13225 as a genospecies of Diaphorobacter.