Experimental primers containing synthetic and natural compounds reduce enzymatic activity at the dentin-adhesive interface under cyclic loading
Experimental primers containing synthetic and natural compounds reduce enzymatic activity at the dentin-adhesive interface under cyclic loading
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DOI：
10.1016/j.dental.2016.07.012
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发表时间：
2016-10-01
期刊：
影响因子：
5
通讯作者：
Bedran-Russo, Ana K.
中科院分区：
文献类型：
--
作者：
Silva Sousa, Ana Beatriz;Vidal, Cristina M. P.;Bedran-Russo, Ana K.
目标。评价实验引物（氯己定、原花青素和强力霉素的富集混合物）对ⅰ类咬合修复体牙本质-树脂界面黏附性能和溶胶活性的影响。用荧光法和明胶酶谱法评价引物对酶的失活作用。为了评估粘合性能，我们在健全的人磨牙上制备了咬合I类牙槽，用磷酸蚀刻，用一种底物和一种蚀刻-冲洗粘合剂系统（Adper Single Bond Plus-3M ESPE）修复。修复后，将标本分为两组（n = 6），分别存放在孵育缓冲液中或在50 n和1,000,000次循环下进行轴向循环加载。然后，对样品进行切片，切片进行原位酶谱分析和微拉伸结合强度（TBS）测试。荧光分析和明胶酶谱分析显示，实验引物灭活了rmmp。原位酶谱分析（2-way ANOVA, Tukey, p < 0.05）表明，循环加载增加了树脂-牙本质界面的凝胶溶解活性，实验引物降低了粘合剂界面的凝胶溶解活性。实验引物对牙本质黏结强度无显著影响（2-way方差分析，p < 0.05）。实验引物的使用破坏了循环加载后牙本质-粘接界面的酶活性和rmmp的活性。循环加载对粘结强度无显著影响。(C) 2016年牙科材料学会。Elsevier Ltd.出版。版权所有。
Objective. To evaluate the effect of experimental primers (chlorhexidine, enriched mixture of proanthocyanidins, and doxycycline) on the adhesive properties and gelatinolytic activity at dentin-resin interfaces of occlusal Class I restorations.Methods. The inactivation of enzymes by the experimental primers was assessed by fluorescence assay and gelatin zymography. To assess the adhesive properties, occlusal Class I cavities were prepared in sound human molars, etched with phosphoric acid and restored with one of the primers and an etch-and-rinse adhesive system (Adper Single Bond Plus-3M ESPE). After the restorative procedures, specimens were divided into two subgroups (n = 6) consisting of storage in incubation buffer or axial cyclic loading at 50 N and 1,000,000 cycles. Then, the specimens were sectioned and slices were assigned to in situ zymography assay and microtensile bond strength (TBS) test.Results. Fluorescence assay and gelatin zymography revealed that the experimental primers inactivated rMMPs. In situ zymography (2-way ANOVA, Tukey, p < 0.05) showed that cyclic loading increased the gelatinolytic activity at the resin-dentin interface and the experimental primers decreased the gelatinolytic activity at the adhesive interface. The experimental primers had no significant effects on dentin-adhesive bond strengths with or without cyclic loading (2-way ANOVA, p > 0.05).Significance. The use of experimental primers impaired the enzymatic activity at the dentin-adhesive interface after cyclic loading and the activity of rMMPs. Cyclic loading did not have a significant effect on the bond strength. (C) 2016 The Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.