Single-copy sequence hybridizes to polymorphic and homologous loci on human X and Y chromosomes.
Single-copy sequence hybridizes to polymorphic and homologous loci on human X and Y chromosomes.
复制标题
单拷贝序列与人类 X 和 Y 染色体上的多态性和同源基因座杂交。
DOI：
10.1073/pnas.79.17.5352
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发表时间：
1982
影响因子：
11.1
通讯作者：
Botstein,D
中科院分区：
文献类型：
--
作者：
Page,D;deMartinville,B;Barker,D;Wyman,A;White,R;Francke,U;Botstein,D
使用来自人类基因组文库的单拷贝DNA的4.5-内切酶对（kb）片段作为人类基因组DNA的杂交探针，揭示了10.6、11.8和14.6kb长的等位基因Taq I限制性片段。在12个无关个体中，除了一个其他片段外，所有6个雄性都显示出14.6-kb片段。其中三只雌性显示10.6-和11.8-kb片段，其他三只显示只有一个片段长度;没有一只显示14.6-kb片段。将该探针与来自人-啮齿动物杂交细胞系（其具有人染色体的部分互补物）的Taq I消化的DNA杂交，证明了14.6-kb片段与人Y染色体的分离以及10.6-和11.8-kb片段与人X染色体的分离。此外，该探针与来自单一家族的48个成员的Taq I消化的DNA杂交，揭示了14.6-kb片段的Y连锁遗传和10.6-和11.8-kb片段的X连锁遗传。这些实验证明了人类X和Y染色体上的单拷贝序列之间的同源性。
Use of a 4.5-kilobase-pair (kb) segment of single-copy DNA from a human genomic library as a hybridization probe of genomic human DNAs revealed allelic Taq I restriction fragments 10.6, 11.8, and 14.6 kb long. Among 12 unrelated individuals, all 6 males exhibited the 14.6-kb fragment in addition to one of the other fragments. Three of the females displayed 10.6- and 11.8-kb fragments, and the other three displayed only one fragment length; none had the 14.6-kb fragment. Hybridization of this probe to Taq I-digested DNAs from human-rodent hybrid cell lines (which have partial complements of human chromosomes) demonstrated segregation of the 14.6-kb fragment with the human Y chromosome and segregation of the 10.6- and 11.8-kb fragments with the human X chromosome. Furthermore, hybridization of this probe to Taq I-digested DNAs from 48 members of a single kindred revealed Y-linked inheritance of the 14.6-kb fragment and X-linked inheritance of the 10.6- and 11.8-kb fragments. These experiments demonstrate homology between single-copy sequences on the human X and Y chromosomes.