Improvement of the catalytic characteristics of a salt-tolerant GH10 xylanase from Streptomyce rochei L10904
Improvement of the catalytic characteristics of a salt-tolerant GH10 xylanase from Streptomyce rochei L10904
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罗氏链霉菌 L10904 耐盐 GH10 木聚糖酶催化特性的改进
DOI：
10.1016/j.ijbiomac.2017.10.013
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发表时间：
2018-02-01
影响因子：
8.2
通讯作者：
Teng, Chao
中科院分区：
文献类型：
--
作者：
Li, Qin;Sun, Baoguo;Teng, Chao
研究了罗氏链霉菌（Streptomycesrochei）L10904产生的GH 10木聚糖酶Srxyn 10及其截短衍生物Srxyn 10 M。两者都表现出很强的耐盐能力，在37 ℃下在3.0 M和5.0 M NaCl中孵育1 h后，分别保留了95%和91%以上的活性。它们表现出一种特殊的水解性能，形成木二糖作为主要产物，并产生较少的木糖化合物时，与一种已报道的木聚糖酶结合，同时消化玉米芯木聚糖。突变体，Srxyn 10 M，构建从Srxyn 10通过删除C-末端碳水化合物结合模块。它对山毛榉木聚糖的比活性比Srxyn 10高3.26倍。此外，Srxyn 10 M表现出更大的底物亲和力和催化效率比Srxyn 10时，山毛榉木聚糖，桦木木聚糖，燕麦-斯佩耳特木聚糖用作底物。热稳定性也大大提高。因此，这些性能的改善显着提高了应用潜力。(C)2017爱思唯尔B. V.保留所有权利。
A GH10 xylanase Srxyn10 from Streptomyce rochei L10904, and its truncated derivative, Srxyn10M, were investigated. Both displayed great salt-tolerant ability, retaining more than 95% and 91% activity after incubation at 37 degrees C for 1 h in 3.0 M and 5.0 M NaCl, respectively. They exhibited a special hydrolytic property of forming xylobiose as the major product and produced fewer xylose compounds when combined with a reported xylanase while digesting corncob xylans. The mutant, Srxyn10M, was constructed from Srxyn10 by deleting the C-terminal carbohydrate-binding module. It possessed a 3.26-fold higher specific activity on beechwood xylan than Srxyn10. Moreover, Srxyn10M showed greater substrate affinity and catalytic efficiency than Srxyn10 when beechwood xylan, birchwood xylan, and oat-spelt xylan were used as substrates. The thermostability was also greatly improved. Therefore, the application potential was markedly enhanced by the improvement of these properties. (C) 2017 Elsevier B.V. All rights reserved.