Universal probe-based SNP genotyping with visual readout: a robust and versatile method
Universal probe-based SNP genotyping with visual readout: a robust and versatile method
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DOI：
10.1039/d3nr01950k
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发表时间：
2023-07-07
期刊：
影响因子：
6.7
通讯作者：
Xie，Guoming
中科院分区：
文献类型：
--
作者：
Wang，Zhongzhong;Zhang，Zhang;Xie，Guoming
检测单核苷酸多态性（snp）对于个性化临床诊断、治疗和药物治疗至关重要。目前的临床检测方法受到引物二聚化的影响，需要针对不同的靶点重新设计反应系统，这是一个费时费力的过程。在这里，我们提出了一种强大而通用的SNP分型方法，通过使用尾部引物和通用小分子探针结合可视化横向流动试验（LFA）。该方法不仅可以快速分型不同的靶点，而且消除了引物二聚体的干扰，提高了结果的准确性和可靠性。我们提出的通用检测方法已成功应用于临床样品的4个SNP位点的分型，验证了其准确性和普适性，结果与Sanger测序结果一致。因此，我们的研究建立了一个新的通用的核酸标签和小分子探针“分型公式”，为遗传分析和分子诊断提供了一个强大的基因分型平台。
Detection of single nucleotide polymorphisms (SNPs) is critical for personalized clinical diagnosis, treatment, and medication. Current clinical detection methods suffer from primer dimerization and require the redesigning of reaction systems for different targets, resulting in a time-consuming and laborious process. Here, we present a robust and versatile method for SNP typing by using tailed primers and universal small molecule probes in combination with a visualized lateral flow assay (LFA). This approach enables not only rapid typing of different targets, but also eliminates the interference of primer dimers and enhances the accuracy and reliability of the results. Our proposed universal assay has been successfully applied to the typing of four SNP loci of clinical samples to verify the accuracy and universality, and the results are consistent with those obtained by Sanger sequencing. Therefore, our study establishes a new universal “typing formula” using nucleic acid tags and small molecule probes that provides a powerful genotyping platform for genetic analysis and molecular diagnostics.