CHROMOSOME LENGTH CONTROLS MITOTIC CHROMOSOME SEGREGATION IN YEAST
CHROMOSOME LENGTH CONTROLS MITOTIC CHROMOSOME SEGREGATION IN YEAST
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DOI：
10.1016/0092-8674(86)90284-9
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发表时间：
1986-05-23
期刊：
影响因子：
64.5
通讯作者：
SZOSTAK, JW
中科院分区：
文献类型：
--
作者：
MURRAY, AW;SCHULTES, NP;SZOSTAK, JW
我们研究了物理长度对人工染色体和天然酵母染色体片段的有丝分裂分离的影响。增加人工染色体的长度可以降低它们在有丝分裂过程中丢失的速度。我们通过在染色体长度的沿着不同位置整合新的端粒，制造了第三号染色体的片段。42和72 kb的染色体片段表现得像人工染色体：它们在有丝分裂中比自然染色体更频繁地丢失。相比之下，150 kb的染色体片段在有丝分裂中与其来源的全长染色体一样稳定。短双着丝粒人工染色体的结构不稳定性表明，尽管短人工染色体在有丝分裂中分离较差，但它们确实附着在有丝分裂纺锤体上。我们讨论了这些结果的背景下，在该模型中，染色体分离是由分离的DNA分子的连锁。
We have examined the effect of physical length on the mitotic segregation of artificial chromosomes and fragments of natural yeast chromosomes. Increasing the length of artificial chromosomes decreases the rate at which they are lost during mitosis. We have made fragments of chromosome III by integrating new telomeres at different positions along the length of the chromosome. Chromosome fragments of 42 and 72 kb behave like artificial chromosomes: they are lost in mitosis much more frequently than natural chromosomes. In contrast, a chromosome fragment of 150 kb is as mitotically stable as the full-length chromosome from which it is derived. The structural instability of a short dicentric artificial chromosome demonstrates that, although short artificial chromosomes segregate poorly in mitosis, they do attach to the mitotic spindle. We discuss these results in the context of a model in which chromosome segregation is directed by the intercatenation of the segregating DNA molecules.