Targeted, LCMS-based Metabolomics for Quantitative Measurement of NAD(+) Metabolites.
Targeted, LCMS-based Metabolomics for Quantitative Measurement of NAD(+) Metabolites.
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DOI：
10.5936/csbj.201301012
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发表时间：
2013
影响因子：
6
通讯作者：
Brenner C
中科院分区：
文献类型：
--
作者：
Trammell SA;Brenner C
Nicotinamide adenine dinucleotide (NAD+) is a coenzyme for hydride transfer reactions and a substrate for sirtuins and other NAD+-consuming enzymes. The abundance of NAD +, NAD+ biosynthetic intermediates, and related nucleotides reflects the metabolic state of cells and tissues. High performance liquid chromatography (HPLC) followed by ultraviolet-visible (UV-Vis) spectroscopic analysis of NAD+ metabolites does not offer the specificity and sensitivity necessary for robust quantification of complex samples. Thus, we developed a targeted, quantitative assay of the NAD+ metabolome with the use of HPLC coupled to mass spectrometry. Here we discuss NAD+ metabolism as well as the technical challenges required for reliable quantification of the NAD+ metabolites. The new method incorporates new separations and improves upon a previously published method that suffered from the problem of ionization suppression for particular compounds.
