The analysis of streptococcal infections. V. Cardiotoxicity of streptolysin O for rabbits in vivo.
The analysis of streptococcal infections. V. Cardiotoxicity of streptolysin O for rabbits in vivo.
复制标题
链球菌感染分析。
DOI：
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发表时间：
1961
期刊：
影响因子：
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通讯作者：
E. Dahle
中科院分区：
文献类型：
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作者：
S. Halbert;R. Bircher;E. Dahle
1.静脉注射活化的链球菌溶血素O后出现的快速死亡。A组或C组链球菌感染之前总是有深刻的心电图改变。在几倍的LD 50剂量后，在注射完成后2至4秒内可能发生心脏电骤停或纤颤。2.所用的链球菌溶血素O制剂是相当高纯度的，但已知被少量的一种或两种其他免疫学上不同的组分污染。通过可逆氧化材料试验、胆固醇灭活以及富含抗链球菌溶血素抗体的人丙种球蛋白的体内保护，获得了观察到的结果是由于链球菌溶血素O所致的证据。3.四个非链球菌溶血素链球菌抗原，部分或高度纯化，未能产生类似的心电图变化，毒性小得多。其中之一是3X重结晶和1X再层析的链球菌蛋白酶。副痢疾杆菌III型内毒素也没有产生明显的心电图异常。4.一个工作假说已经开发牵连链球菌溶血素O的致病性链球菌因子负责风湿热的发病机制，这似乎占这种疾病的主要特点。
1. The rapid death which occurred after intravenous injection of activated streptolysin O from. Group A or Group C streptococci was always preceded by profound electrocardiographic alterations. After several multiples of the LD50 doses, cardiac electrical arrest or fibrillation could occur within 2 to 4 seconds after completion of the injection. 2. The streptolysin O preparations used were rather highly purified, but were known to be contaminated with small amounts of one or two other immunologically distinct components. Evidence that the observed results were due to streptolysin O was obtained by tests of the reversibly oxidized materials, and by cholesterol inactivation, as well as by in vivo protection with human gamma globulin rich in antistreptolysin antibodies. 3. Four non-streptolysin streptococcal antigens, partially or highly purified, failed to produce similar electrocardiographic changes, and were much less toxic. One of these was 3 X recrystallized and 1 X rechromatographed streptococcal proteinase. Shigella paradysenteriae type III endotoxin also did not produce striking electrocardiographic abnormalities. 4. A working hypothesis has been developed implicating streptolysin O as the etiological streptococcal factor responsible for the pathogenesis of rheumatic fever, which seems to account for the principal features of this illness.