THE ORGANIZATION OF THE FUC REGULON SPECIFYING L-FUCOSE DISSIMILATION IN ESCHERICHIA-COLI-K12 AS DETERMINED BY GENE CLONING
THE ORGANIZATION OF THE FUC REGULON SPECIFYING L-FUCOSE DISSIMILATION IN ESCHERICHIA-COLI-K12 AS DETERMINED BY GENE CLONING
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DOI：
10.1007/bf00325702
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发表时间：
1987-12-01
期刊：
影响因子：
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通讯作者：
LIN, ECC
中科院分区：
文献类型：
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作者：
CHEN, YM;ZHU, Y;LIN, ECC
在大肠杆菌中，指定利用L-岩藻糖作为碳源和能源的六个已知基因在60，2分钟聚集并构成调节子。这些基因包括fucP（编码L-岩藻糖通透酶）、fucI（编码L-岩藻糖异构酶）、fucK（编码L-岩藻糖激酶）、fucA（编码L-岩藻糖1-磷酸醛缩酶）、fucO（编码L-1，2-丙二醇氧化还原酶）和fucR（编码调节蛋白）。本研究克隆了fuc基因，并通过限制性内切酶和互补分析确定了它们在染色体上的位置。因此，基因顺序为：fucO-fucA-fucP-fucI-fucK-fucR。通过互补分析和Southern印迹杂交确定了构成结构基因的操纵子和转录方向。fucPIK和fucA操纵子顺时针转录。fucO操纵子逆时针转录。fucR基因产物以反式激活三个结构操纵子。
In Escherichia coli the six known genes specifying the utilization of L-fucose as carbon and energy source cluster at 60,2 min and constitute a regulon. These genes include fucP (encoding L-fucose permease), fucI (encoding L-fucose isomerase), fucK (encoding L-fuculose kinase), fucA (encoding L-fuculose 1-phosphate aldolase), fucO (encoding L-1,2-propanediol oxidoreductase), and fucR (encoding the regulatory protein). In this study the fuc genes were cloned and their positions on the chromosome were established by restriction endonuclease and complementation analyses. Clockwise, the gene order is: fucO-fucA-fucP-fucI-fucK-fucR. The operons comprising the structural genes and the direction of transcription were determined by complementation analysis and Southern blot hybridization. The fucPIK and fucA operons are transcribed clockwise. The fucO operon is transcribed counterclockwise. The fucR gene product activates the three structural operons in trans.