Curcuminoids and sesquiterpenoids in turmeric (Curcuma longa L.) suppress an increase in blood glucose level in type 2 diabetic KK-Ay mice
Curcuminoids and sesquiterpenoids in turmeric (Curcuma longa L.) suppress an increase in blood glucose level in type 2 diabetic KK-Ay mice
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DOI：
10.1021/jf0483873
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发表时间：
2005-02-23
影响因子：
6.1
通讯作者：
Kitahara, M
中科院分区：
文献类型：
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作者：
Nishiyama, T;Mae, T;Kitahara, M
姜黄是姜黄科植物姜黄（Curcuma longa L.）的根茎，对人体健康具有广泛的作用。该化学成分包括姜黄素和倍半萜类化合物，已知它们具有抗氧化、抗癌和抗炎活性。在这项研究中，我们研究了三种姜黄提取物对 2 型糖尿病 KK-A(y) 小鼠（6 周龄，n = 5/组）血糖水平的影响。这些姜黄提取物通过乙醇提取（E-ext）获得类姜黄素和倍半萜类化合物，通过己烷提取（H-ext）获得倍半萜类化合物，并从己烷提取残渣中进行乙醇提取（HE-ext）以获得类姜黄素。对照组饲喂基础饮食，而其他组饲喂含有0.1或0.5g H-ext或HE-ext/100g饮食或0.2或1.0g E-ext/100g饮食的饮食，持续4周。尽管4周后对照组的血糖水平显着升高（P < 0.01），但每100 g饮食添加0.2或1.0 g E-ext、0.5 g H-ext和0.5 g HE-ext可抑制血糖水平的显着升高。此外，E-ext 刺激人脂肪细胞分化，并且在 GAL4-PPAR-gamma 嵌合体测定中，这些姜黄提取物具有人过氧化物酶体增殖物激活受体-gamma (PPAR-gamma) 配体结合活性。此外，姜黄素、去甲氧基姜黄素、双去甲氧基姜黄素和蒿甲酮具有 PPAR-γ 配体结合活性。这些结果表明，姜黄中的类姜黄素和倍半萜类化合物均通过作为机制之一的PPAR-γ激活而表现出降血糖作用，并表明包含类姜黄素和倍半萜类化合物的E-ext具有两种成分的相加或协同作用。
Turmeric, the rhizome of Curcuma longa L., has a wide range of effects on human health. The chemistry includes curcuminoids and sesquiterpenoids as components, which are known to have antioxidative, anticarcinogenic, and antiinflammatory activities. In this study, we investigated the effects of three turmeric extracts on blood glucose levels in type 2 diabetic KK-A(y) mice (6 weeks old, n = 5/group). These turmeric extracts were obtained by ethanol extraction (E-ext) to yield both curcuminoids and sesquiterpenoids, hexane extraction (H-ext) to yield sesquiterpenoids, and ethanol extraction from hexane- extraction residue (HE-ext) to yield curcuminoids. The control group was fed a basal diet, while the other groups were fed a diet containing 0.1 or 0.5 g of H-ext or HE-ext/100 g of diet or 0.2 or 1.0 g of E-ext/100 g of diet for 4 weeks. Although blood glucose levels in the control group significantly increased (P < 0.01) after 4 weeks, feeding of 0.2 or 1.0 g of E-ext, 0.5 g of H-ext, and 0.5 g of HE-ext/100 g of diet suppressed the significant increase in blood glucose levels. Furthermore, E-ext stimulated human adipocyte differentiation, and these turmeric extracts had human peroxisome proliferator-activated receptor-gamma (PPAR-gamma) ligand-binding activity in a GAL4-PPAR-gamma chimera assay. Also, curcumin, demethoxycurcumin, bisdemethoxycurcumin, and arturmerone had PPAR-gamma ligand-binding activity. These results indicate that both curcuminoids and sesquiterpenoids in turmeric exhibit hypoglycemic effects via PPAR-gamma activation as one of the mechanisms, and suggest that E-ext including curcuminoids and sesquiterpenoids has the additive or synergistic effects of both components.