INHIBITORS OF CA2+ CHANNELS, CALMODULIN AND PROTEIN-KINASES PREVENT A23187 AND OTHER INDUCTIONS OF METALLOTHIONEIN MESSENGER-RNA IN EC3 RAT HEPATOMA-CELLS
INHIBITORS OF CA2+ CHANNELS, CALMODULIN AND PROTEIN-KINASES PREVENT A23187 AND OTHER INDUCTIONS OF METALLOTHIONEIN MESSENGER-RNA IN EC3 RAT HEPATOMA-CELLS
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DOI：
10.1016/0024-3205(93)90126-n
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发表时间：
1993-01-01
期刊：
影响因子：
6.1
通讯作者：
BRADY, FO
中科院分区：
文献类型：
--
作者：
ARIZONO, K;PETERSON, KL;BRADY, FO
在EC 3大鼠肝癌细胞中，使用各种诱导剂（A23187、TPA、去甲肾上腺素和2-氯腺苷）和抑制剂（H7：PK-A和PK-C; W7：钙调素;维拉帕米：钙通道阻滞剂;和TMB-8：胞质钙螯合剂）研究了钙在MT mRNA诱导中的作用。 本研究中观察到的诱导抑制与钙本身在MT mRNA诱导中以及通过PK-A、PK-C和钙调蛋白依赖性蛋白激酶途径之间的串扰发挥重要作用一致。钙离子在复杂的第二信使通路中起着重要作用，这些通路导致转录因子与MT基因启动子的正向相互作用。
The role of calcium in the induction of MT mRNA has been studied in EC3 rat hepatoma cells, using various inducers (A23187, TPA, norepinephrine, and 2-chloroadenosine) and inhibitors (H7:PK-A and PK-C; W7:calmodulin; verapamil: calcium channel blocker; and TMB-8; cytosolic calcium chelator). The inhibitions of inductions observed in this study were consistent with calcium playing an important role in MT mRNA induction by itself and via crosstalk among the PK-A, PK-C, and calmodulin- dependent protein kinase pathways. Calcium has an important role in the complicated second messenger pathways which result in the positive interaction of transcription factors with the promoters of MT genes.