Ectopic 5' splice sites inhibit gene expression by engaging RNA surveillance and silencing pathways in plants.
Ectopic 5' splice sites inhibit gene expression by engaging RNA surveillance and silencing pathways in plants.
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异位 5 剪接位点通过参与植物中的 RNA 监视和沉默途径来抑制基因表达。
DOI：
10.1104/pp.109.139733
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发表时间：
2009
期刊：
影响因子：
7.4
通讯作者：
Lacomme,Christophe
中科院分区：
文献类型：
--
作者：
Wypijewski,Krzysztof;Hornyik,Csaba;Shaw,JaneA;Stephens,Jennifer;Goraczniak,Rafal;Gunderson,SamuelI;Lacomme,Christophe
mRNA成熟的质量控制是一个高度调节的过程，其调查前mRNA的完整性并消除不适当成熟的前mRNA。在自然界中，某些病毒通过劫持内源性RNA质量控制机制来调节其基因的表达。我们证明了在植物中报告基因的3′-非翻译区中包含5′剪接位点改变了前体mRNA的切割和多聚腺苷酸化过程，导致前体mRNA的降解，例证了王国之间保守的调节机制。改变前mRNA加工与抑制同源基因表达的反式和优先积累的24个核苷酸（nt）的短干扰RNA（siRNA），而不是21-nt的siRNA亚种，这表明降解的异常前mRNA涉及沉默机制。然而，基因表达没有恢复沉默抑制基因的共表达或在RNA依赖性RNA聚合酶（RDR 6）缺陷的背景下，尽管减少24-nt siRNA的积累。我们的数据突出了质量控制RNA机制，3′-末端前mRNA成熟和RNA沉默途径之间的复杂串扰，能够区分不同类型的异常RNA。
The quality control of mRNA maturation is a highly regulated process that surveys pre-mRNA integrity and eliminates improperly matured pre-mRNAs. In nature, certain viruses regulate the expression of their genes by hijacking the endogenous RNA quality control machinery. We demonstrate that the inclusion of 5′ splice sites within the 3′-untranslated region of a reporter gene in plants alters the pre-mRNA cleavage and polyadenylation process, resulting in pre-mRNA degradation, exemplifying a regulatory mechanism conserved between kingdoms. Altered pre-mRNA processing was associated with an inhibition of homologous gene expression in trans and the preferential accumulation of 24-nucleotide (nt) short-interfering RNAs (siRNAs) as opposed to 21-nt siRNA subspecies, suggesting that degradation of the aberrant pre-mRNA involves the silencing machinery. However, gene expression was not restored by coexpression of a silencing suppressor or in an RNA-dependent RNA polymerase (RDR6)-deficient background despite reduced 24-nt siRNA accumulation. Our data highlight a complex cross talk between the quality control RNA machinery, 3′-end pre-mRNA maturation, and RNA-silencing pathways capable of discriminating among different types of aberrant RNAs.