Significance of rpoS during maturation of Escherichia coli biofilms
Significance of rpoS during maturation of Escherichia coli biofilms
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DOI：
10.1002/bit.21695
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发表时间：
2008-04-15
影响因子：
3.8
通讯作者：
Okabe, Satoshi
中科院分区：
文献类型：
--
作者：
Ito, Akinobu;May, Thithiwat;Okabe, Satoshi
生物膜中饥饿、静止的相状区的存在似乎是生物膜形成的一个重要因素。本研究探讨了rpos基因在大肠杆菌生物被膜形成中的作用。以野生型(WT)和rpos突变型(Delta Rpos)菌株为研究对象，比较两种菌株的生物被膜形成能力和整体基因表达。尽管Delta rpos菌株可以在玻璃表面附着并形成微菌落，但它不能建立成熟的生物膜。DNA芯片分析表明，WT生物膜(WBF)的基因表达模式与WT浮游稳定期相似，Delta rpos生物膜(MBF)的基因表达模式与WT浮游指数期相似。与能量代谢相关的基因(arpIBEFHAG、atpC、cydAB)和鞭毛合成相关基因(flgB、flgC、flhD、fliA、flic、fliY)在体液中表达增加，而在WBF中没有表达。此外，与应激反应相关的基因(BLC、CSPG、dind poxB、wcaF、wca1和yfcf)在WBF中的表达高于MBF。这些结果表明，rpos基因通过调节包括能量代谢、运动和应激反应在内的整体基因表达，促进了大肠杆菌生物膜的成熟。
Presence of starved, stationary phase-like zones in biofilms seems to be an important factor for biofilm formation. In this study, roles of rpoS gene in the formation of Escherichia coli biofilms were investigated. E. coli MG 1655 wild type (WT) and rpoS mutant (Delta rpoS) strains were used to compare biofilm formation capacity and global gene expression. Even though the Delta rpoS strain could attach and form microcolonies on glass surfaces, it could not establish mature biofilms. DNA microarray analysis revealed that WT biofilms (WBF) showed similar pattern of gene expression with WT planktonic stationary phase, whereas Delta rpoS biofilms (MBF) showed similar pattern of gene expression with WT planktonic exponential phase. Genes involved in energy metabolism (arpIBEFHAG, atpC, cydAB) and flagella synthesis (flgB, flgC, flhD, fliA, fliC, fliY) showed increased expression in the MBF, but not in the WBF. Moreover, genes involved in stress responses (blc, cspG, dinD poxB, wcaF, wcaI, and yfcf) showed increased expression in the WBF compared to the MBF. These results suggested that the rpoS gene contributed in maturation of E. coli biofilms through regulation of global gene expression including energy metabolism, motility, and stress responses.