Age‐Related Changes in Human Blood Dendritic Cell Subpopulations
Age‐Related Changes in Human Blood Dendritic Cell Subpopulations
复制标题
人类血液树突状细胞亚群与年龄相关的变化
DOI：
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发表时间：
2002
影响因子：
3.7
通讯作者：
U. Schauer
中科院分区：
文献类型：
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作者：
N. Teig;D. Moses;S. Gieseler;U. Schauer
众所周知，人类免疫系统在出生时功能不太成熟，在生命的最初几年里，经历了一个循序渐进的发展过程。在血液中发现了两种树突状细胞亚群：浆细胞样和髓细胞样树突状细胞。树突状细胞CD3、CD14、CD16、CD19、CD20和CD56呈阴性，人白细胞抗原（HLA）‐DR呈阳性。此外，浆细胞样dc强烈表达CD123，而髓细胞样dc表达CD11。通过流式细胞术检测44例8个月至18岁的健康婴儿和儿童外周血中这两种不同亚群的数量。随着年龄的增长，CD11c+髓样细胞的数量（平均11个细胞/µl）没有变化，而CD123+浆细胞样dc的比例显著下降（P < 0.001），从约20个细胞/µl下降到8个细胞/µl。此外，我们发现浆细胞样DC的数量与干扰素(IFN) α的产生有直接关系。由于DC的两个亚群优先识别不同的病原体，年龄相关的变化可能会影响免疫反应的成熟。
It is well known that the human immune system is functionally less mature at birth and, within the first years of life, undergoes a process of sequential development. Two subsets of dendritic cells (DCs) were identified in the blood: plasmacytoid and myeloid DCs. DCs stain negative for CD3, CD14, CD16, CD19, CD20 and CD56, and positive for human leucocyte antigen (HLA)‐DR. In addition, plasmacytoid DCs strongly express CD123 while myeloid DCs express CD11. The number of the two different subsets was measured by flow cytometry in the peripheral blood from 44 healthy infants and children, aged 8 months to 18 years. While the number of CD11c+ myeloid cells (mean 11 cells/µl) did not change with age, the proportion of CD123+ plasmacytoid DCs decreased significantly (P < 0.001) from about 20 cells/µl to 8 cells/µl with age. In addition, we found a direct correlation between the number of plasmacytoid DC and interferon (IFN)‐α production. As the two subsets of DC preferentially recognize different pathogens, age‐related changes may have an impact on the maturation of the immune response.
DOI：
10.1073/pnas.96.16.9305
发表时间：
1999-08-03
影响因子：
11.1
作者：
Hartmann, G;Weiner, GJ;Krieg, AM
通讯作者：
Krieg, AM