Epithelial cell adhesion in the developing Drosophila retina is regulated by Atonal and the EGF receptor pathway
Epithelial cell adhesion in the developing Drosophila retina is regulated by Atonal and the EGF receptor pathway
复制标题
DOI：
10.1016/j.ydbio.2006.08.003
复制
发表时间：
2006-12-15
影响因子：
2.7
通讯作者：
Freeman, Matthew
中科院分区：
文献类型：
--
作者：
Brown, Katherine E.;Baonza, Antonio;Freeman, Matthew
在果蝇视网膜中，感光细胞分化之前，在形态发生沟内和紧接在形态发生沟后面的显著的细胞形状重排。细胞群在上皮平面上聚集成弧形和圆形，神经元随后从中出现。这些细胞簇也具有不同的粘附特性：粘附连接组分相对于周围细胞上调。关于这些形态学变化是如何协调的以及它们与随后的神经元分化的相关性知之甚少。在这里，我们报告说，转录因子Atonal和经典的EGF受体信号级联都是这种聚集和伴随的细胞粘附变化所必需的。在没有任何一种成分的情况下，在沟后面没有形成弧，并且所有细胞都显示出低的Armadillo和DE-钙粘蛋白水平，尽管在EGFR途径突变体的情况下，可以看到具有高水平粘附连接成分的单个推定R8细胞。无调调节DE-钙粘蛋白的转录，而EGFR途径，通过转录因子点，发挥其作用的粘附连接间接，在转录后水平。这些观察结果定义了一个新的功能，EGFR信号在眼睛的发展，并说明了一种机制，控制上皮形态的发展信号。(c)2006年爱思唯尔公司All rights reserved.
In the Drosophila retina, photoreceptor differentiation is preceded by significant cell shape rearrangements within and immediately behind the morphogenetic furrow. Groups of cells become clustered into arcs and rosettes in the plane of the epithelium, from which the neurons subsequently emerge. These cell clusters also have differential adhesive properties: adherens junction components are upregulated relative to surrounding cells. Little is known about how these morphological changes are orchestrated and what their relevance is for subsequent neuronal differentiation. Here, we report that the transcription factor Atonal and the canonical EGF receptor signalling cascade are both required for this clustering and for the accompanying changes in cellular adhesion. In the absence of either component, no arcs are formed behind the furrow, and all cells show low Armadillo and DE-cadherin levels, although in the case of EGFR pathway mutants, single, presumptive R8 cells with high levels of adherens junction components can be seen. Atonal regulates DE-cadherin transcriptionally, whereas the EGFR pathway, acting through the transcription factor Pointed, exerts its effects on adherens junctions indirectly, at a post-transcriptional level. These observations define a new function for EGFR signalling in eye development and illustrate a mechanism for the control of epithelial morphology by developmental signals. (c) 2006 Elsevier Inc. All rights reserved.