Bone architecture and disc degeneration in the lumbar spine of mice lacking GDF-8 (myostatin)
Bone architecture and disc degeneration in the lumbar spine of mice lacking GDF-8 (myostatin)
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DOI：
10.1016/s0736-0266(03)00105-0
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发表时间：
2003-11-01
影响因子：
2.8
通讯作者：
Byron, CD
中科院分区：
文献类型：
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作者：
Hamrick, MW;Pennington, C;Byron, CD
GDF-8，也称为肌生长抑制素，是在脊椎动物骨骼肌中表达的分泌性生长和分化因子的转化生长因子-β超家族的成员。肌生长抑制素作为骨骼肌生长的负调节剂发挥作用，并且肌生长抑制素缺失小鼠显示出与正常小鼠相比肌肉质量加倍。我们在这里描述的形态学的腰椎肌肉生长抑制素基因敲除（MSTO（-/-））小鼠使用组织学和光密度技术。在本研究中检查的Mc（-/-）小鼠比对照组重约10%（p < 0.001），但敲除小鼠的髂腰肌比野生型小鼠大50%以上（p < 0.001）。来自第五腰椎的外周定量计算机断层扫描（pQCT）数据显示，缺乏肌生长抑制素的小鼠比正常小鼠具有大约50%的骨小梁矿物质密度（p = 0.001）和显著更高的皮质骨矿物质含量。L4-L5之间椎间盘的甲苯胺蓝染色揭示了敲除小鼠的透明终板和内部纤维环中蛋白聚糖染色的损失。L4尾侧终板软骨染色的丧失是由于肌生长抑制素缺乏动物终板骨化所致。这项研究的结果表明，缺乏肌生长抑制素的小鼠肌肉质量增加与骨量增加以及椎间盘退行性变化有关。(C)2003骨科研究学会。由爱思唯尔有限公司出版。保留所有权利。
GDF-8, also known as myostatin, is a member of the transforming growth factor-beta superfamily of secreted growth and differentiation factors that is expressed in vertebrate skeletal muscle. Myostatin functions as a negative regulator of skeletal Muscle growth and myostatin null mice show a doubling of muscle mass compared to normal mice. We describe here morphology of the lumbar spine in myostatin knockout (Mstn(-/-)) mice using histological and densitometric techniques. The Mstn(-/-) mice examined in this study weigh approximately 10% more than controls (p < 0.001) but the iliopsoas muscle is over 50% larger in the knockout mice than in wild-type mice (p < 0.001). Peripheral quantitative computed tomography (pQCT) data from the fifth lumbar vertebra show that mice lacking myostatin have approximately 50% greater trabecular bone mineral density (p = 0.001) and significantly greater cortical bone mineral content than normal mice. Toluidine blue staining of the intervertebral disc between L4-L5 reveals loss of proteoglycan staining in the hyaline end plates and inner annulus fibrosus of the knockout mice. Loss of cartilage staining in the caudal end plate of L4 is due to ossification of the end plate in the myostatin-deficient animals. Results from this study suggest that increased muscle mass in mice lacking myostatin is associated with increased bone mass as well as degenerative changes in the intervertebral disc. (C) 2003 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.