Isolation of mutants defective in early steps of meiotic recombination in the yeast Saccharomyces cerevisiae.
Isolation of mutants defective in early steps of meiotic recombination in the yeast Saccharomyces cerevisiae.
复制标题
酿酒酵母减数分裂重组早期缺陷突变体的分离。
DOI：
10.1093/genetics/128.1.79
复制
发表时间：
1991
期刊：
影响因子：
3.3
通讯作者：
Galbraith,A
中科院分区：
文献类型：
--
作者：
Malone,RE;Bullard,S;Hermiston,M;Rieger,R;Cool,M;Galbraith,A
使用基于早期Rec突变的能力的选择（例如，RAD 50）为了挽救RAD 52 Spo 13菌株的减数分裂致死性，我们分离了177个突变体。对其中56个的分析产生了已知Rec基因SPO 11、ME 14和MER 1的等位基因，并定义了5个新基因：REC 102、REC 104、REC 107、REC 113和REC 114。所有新基因的突变似乎都特异性地影响减数分裂;它们没有任何可检测到的有丝分裂表型。REC 102、REC 104和REC 107中的突变使减数分裂重组减少数百倍。没有分离到RED 1或HOP 1的等位基因，这与这些基因可能主要参与染色体配对而不是交换的提议一致。
Using a selection based upon the ability of early Rec- mutations (e.g., rad50) to rescue the meiotic lethality of a rad52 spo13 strain, we have isolated 177 mutants. Analysis of 56 of these has generated alleles of the known Rec genes SPO11, ME14 and MER1, as well as defining five new genes: REC102, REC104, REC107, REC113 and REC114. Mutations in all of the new genes appear to specifically affect meiosis; they do not have any detectable mitotic phenotype. Mutations in REC102, REC104 and REC107 reduce meiotic recombination several hundred fold. No alleles of RED1 or HOP1 were isolated, consistent with the proposal that these genes may be primarily involved with chromosome pairing and not exchange.