Microparticle association and heterogeneity of tumor-derived tissue factor in plasma: is it important for coagulation activation?
Microparticle association and heterogeneity of tumor-derived tissue factor in plasma: is it important for coagulation activation?
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DOI：
10.1111/jth.12475
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发表时间：
2014-02-01
影响因子：
10.4
通讯作者：
Amirkhosravi, A.
中科院分区：
文献类型：
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作者：
Davila, M.;Robles-Carrillo, L.;Amirkhosravi, A.
背景肿瘤源性组织因子（TF）在人胰腺癌原位模型中激活体外和体内凝血。在这里，我们进一步的特点是肿瘤衍生的TF在这个model. MethodsConditionedmedium（CM）的L3.6pl人胰腺肿瘤细胞和血浆从裸鼠荷L3.6pl肿瘤进行ultracenthuged，并通过不同孔径的膜过滤的小球。用纳米颗粒跟踪和动态光散射分析CM或血浆级分中颗粒的尺寸分布。人TF抗原和活性分别用ELISA和凝血或凝血酶生成测定法在沉淀和上清液中测量。用ELISA法检测人选择性剪接的TF（asTF）。人TF和凝血酶-抗凝血酶复合物（达特）的浓度进行了评估，在小鼠血浆中注射过滤馏分的CM。
BackgroundTumor-derived tissue factor (TF) activates coagulation invitro and invivo in an orthotopic model of human pancreatic cancer. Here, we further characterized tumor-derived TF in this model.MethodsConditioned medium (CM) of L3.6pl human pancreatic tumor cells and plasma from nude mice bearing L3.6pl tumors were ultracentrifuged, and the pellets were filtered through membranes with different pore sizes. The size distribution of particles was analyzed in CM or plasma fractions with nanoparticle tracking and dynamic light scattering. Human TF antigen and activity were measured in pellets and supernatants with ELISA and clotting or thrombin generation assays, respectively. Human alternatively spliced TF (asTF) was measured with ELISA. Human TF and thrombin-antithrombin complex (TAT) concentrations were assessed in plasma of mice injected with filtered fractions of CM.ResultsParticles in both CM and plasma were