Efficient Differentiation of Mycobacterium abscessus Complex Isolates to the Species Level by a Novel PCR-Based Variable-Number Tandem-Repeat Assay
Efficient Differentiation of Mycobacterium abscessus Complex Isolates to the Species Level by a Novel PCR-Based Variable-Number Tandem-Repeat Assay
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DOI：
10.1128/jcm.02318-10
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发表时间：
2011-03-01
影响因子：
9.4
通讯作者：
Shin, Sung Jae
中科院分区：
文献类型：
--
作者：
Choi, Go-Eun;Chang, Chulhun L.;Shin, Sung Jae
建立了一种新的基于可变数目串联重复序列的双重PCR方法，用于区分结核分枝杆菌复合群分离株，并对85株临床分离株进行了评价。通过多靶序列分析证实了测定准确度。双重PCR法是一种一步、可靠、准确的鉴别M.属。
A novel duplex PCR method based on variable-number tandem-repeat targets to discriminate among Mycobacterium abscessus complex isolates was developed and evaluated in 85 clinical isolates. The assay accuracy was confirmed by a multiple-target sequence analysis. The duplex PCR assay is a one-step, reliable, and accurate assay for discriminating M. abscessus species.