Global Transcriptomic Response of Pseudomonas aeruginosa to Chlorhexidine Diacetate
Global Transcriptomic Response of Pseudomonas aeruginosa to Chlorhexidine Diacetate
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DOI：
10.1021/es9015475
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发表时间：
2009-11-01
影响因子：
11.4
通讯作者：
Bentley, William E.
中科院分区：
文献类型：
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作者：
Nde, Chantal W.;Jang, Hyeung-Jin;Bentley, William E.
铜绿假单胞菌与囊性纤维化患者的医院感染和慢性呼吸道感染有关。二醋酸氯己定（CHX）是一种双胍类消毒剂，用于医院、农业和家庭环境中的细菌控制。更好地了解CHX的作用机制和铜绿假单胞菌对CHX的反应将有助于在这些环境中有效利用CHX控制铜绿假单胞菌。本研究提出，第一次，0.008 mM CHX后10和60分钟的铜绿假单胞菌的转录组反应。我们的研究结果表明，两个治疗时间后，膜转运氧化磷酸化，和电子传递基因下调。10分钟后，DNA修复下调，阻断抗菌剂自我促进吸收的oprH基因上调。60 min后，外膜蛋白、鞭毛、菌毛、氧化磷酸化和电子传递基因表达下调，MexCD-OprJ多药外排泵的mexC和mexD基因表达上调。本研究的结果提高了我们对CHX对铜绿假单胞菌作用模式的理解，并为其他双胍类消毒剂的作用机制提供了见解，可用于开发更有效的消毒剂。
Pseudomonas aeruginosa is implicated in nosocomial infections and chronic respiratory infections in cystic fibrosis patients. Chlorhexidine diacetate(CHX) is a biguanide disinfectant used for bacterial control in the hospital and agricultural and domestic environments. Abetter understanding of the mechanism of action of CHX and the resulting response elicited by P. aeruginosa to CHX will facilitate its effective utilization for P. aeruginosa control in these environments. This study presents, for the first time, the transcriptomic response of A aeruginosa to 0.008 mM CHX after 10 and 60 min. Our results reveal that after both treatment times, membrane transport oxidative phosphorylation, and electron transport genes were downregulated. After 10 min, DNA repair was downregulated and the oprH gene that blocks the self-promoted uptake of antimicrobials was upregulated. After 60 min, outer membrane protein, flagellum, pilus, oxidative phosphorylation, and electron transport genes were downregulated, The mexC and mexD genes of the MexCD-OprJ multidrug efflux pump were significantly upregulated after both treatment times. The results of this study improve our understanding of the mode of action of CHX on P. aeruginosa and provide insights into the mechanism of action of other biguanide disinfectants which can be used for the development of more efficient disinfectants.