Ferritin 1 silencing effect in Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) during experimental infection with Ehrlichia canis
Ferritin 1 silencing effect in Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) during experimental infection with Ehrlichia canis
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DOI：
10.1016/j.ttbdis.2016.10.015
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发表时间：
2017-01-01
影响因子：
3.2
通讯作者：
Domingos, Ana
中科院分区：
文献类型：
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作者：
Ferrolho, Joana;Antunes, Sandra;Domingos, Ana
广义血红扇头蜱（Rhipicephalus sanguineus lato（s.l.）是家犬的一种非常常见的体外寄生虫，能够传播几种对人类和兽医有重要意义的病原体。由于缺乏有效的控制措施，蜱虫感染和蜱传疾病（TBD）仍然是一个严重和持续的问题。因此，必须采取新的控制方法。虽然疫苗接种被认为是一种潜在的控制方法，以减少蜱侵扰，没有抗R。血吸虫疫苗是可用的。蜱虫依靠它们的血食来获得营养并达到性成熟，这使它们暴露在大量的铁中。虽然是几个生物过程的必需分子，但其过量可导致氧化应激。铁稳态是在铁结合蛋白（称为铁蛋白）的帮助下实现的，铁蛋白存在于几个蜱组织和发育阶段。这些进化上保守的蛋白质通过以受控的方式储存和释放铁来调节铁稳态。在本研究中，R.通过RNA干扰（RNAi）使暴露于实验性埃里希体感染的成年雌性血红蛋白铁蛋白1基因沉默。本研究的目的是评估这种蛋白质在蜱类摄食、卵巢发育、卵子发生和病原体获得中的作用。我们的数据表明，沉默铁蛋白1改变蜱的能力，正常地饱食，并导致卵巢（OV）和卵母细胞的形态学和组织化学变化。此外，我们的数据显示，没有E。在两个实验组中均发现了犬DNA。确定在关键生物过程中起作用的分子的功能，例如血液消化或生殖，并且可以被认为是潜在的蜱抗原，将有助于改善针对这些体外寄生虫及其所携带的病原体的当前控制措施。(C)2016爱思唯尔有限公司All rights reserved.
Rhipicephalus sanguineus sensu lato (s.l.) is a very common ectoparasite of domestic dogs able to transmit several pathogens of human and veterinary importance. Tick infestations and tick-borne diseases (TBDs) remain a serious and persistent problem, due to the lack of efficient control measures. It is therefore vital that novel approaches to control are pursued. Whilst vaccination is recognised as a potential control method to reduce tick infestation, no anti-R. sanguineus vaccine is available. Ticks depend on their blood meals to obtain nutrients and to achieve sexual maturity, which exposes them to vast amounts of iron. Although an essential molecule for several biological processes, its excess can lead to oxidative stress. Iron homeostasis is achieved with the help of iron-binding proteins called ferritins, among others, present in several tick tissues and developmental stages. These evolutionarily conserved proteins regulate iron homeostasis by storing and releasing iron in a controlled manner. In this study the R. sanguineus ferritin 1 gene was silenced through RNA interference (RNAi) in adult females exposed to an experimental infection with Ehrlichia cants. The purpose of this study was to assess the role of this protein in tick feeding, ovary development, oogenesis, and pathogen acquisition. Our data has shown that silencing ferritin 1 alters tick competence to normally engorge and causes morphologic and histochemical changes in the ovaries (OV) and oocytes. Furthermore, our data revealed that no E. canis DNA was found in either experimental group. Determining the function of molecules that act in key biological processes, such as blood digestion or reproduction, and that could be considered potential tick antigens will contribute towards the improvement of current control measures against these ectoparasites and the pathogens they vector. (C) 2016 Elsevier GmbH. All rights reserved.