The Glutamate Switch Is Present in All Seven Clades of AAA plus Protein
The Glutamate Switch Is Present in All Seven Clades of AAA plus Protein
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DOI：
10.1021/bi9012512
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发表时间：
2009-09-22
期刊：
影响因子：
2.9
通讯作者：
Bell, Stephen D.
中科院分区：
文献类型：
--
作者：
Mogni, Maria Elena;Costa, Alessandro;Bell, Stephen D.
最近的工作在 AAA+ ATP 酶的 7 个进化枝中的 6 个中发现了“谷氨酸开关”。谷氨酸开关的作用是转导有关底物与 ATP 酶活性位点结合的信息。我们提供的生化证据表明，高度保守的苏氨酸残基在复制解旋酶 MCM 中充当谷氨酸开关，因此揭示谷氨酸开关是所有七个 AAA+ 进化枝的共同特征。
Recent work has identified a "glutamate switch" in six of the seven clades of AAA+ ATPases. The glutamate switch acts to transduce information regarding substrate binding to the ATPase active site. We provide biochemical evidence that a highly conserved threonine residue acts as a glutamate switch in the replicative helicase, MCM, and, thus, reveal that the glutamate switch is a feature common to all seven AAA+ clades.