Self-stacking of exfoliated charged nanosheets of LDHs and graphene as biosensor with real-time tracking of dopamine from live cells
Self-stacking of exfoliated charged nanosheets of LDHs and graphene as biosensor with real-time tracking of dopamine from live cells
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DOI：
10.1016/j.aca.2018.10.008
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发表时间：
2019-01-24
影响因子：
6.2
通讯作者：
Liu, Hongfang
中科院分区：
文献类型：
--
作者：
Aziz, Ayesha;Asif, Muhammad;Liu, Hongfang
本研究提出了一种周期性堆积带正电的NiAl层状双氢氧化物(LDHs)纳米片与带负电的石墨烯(G)单分子膜的新策略，通过控制共进料的方式系统地优化了几个参数，并得到了异质堆积的NiAl LDH/GLBL纳米复合材料，作为早期帕金森病(PD)的诊断工具，已被实际应用于灵敏地检测活细胞释放的多巴胺。帕金森病是第二大慢性神经退行性疾病，逐渐丧失运动和肌肉控制，导致严重残疾并严重威胁生命。不幸的是，帕金森病患者黑质中存在的大部分多巴胺能神经元在临床诊断之前就已经被破坏，因此早期诊断是必不可少的。由于极具导电性的石墨烯直接与半导体LDHs层相邻，LDHs的插层能力增强，并且具有巨大的比表面积和众多的活性中心，因此异质组装的NiAl LDH/GLBL材料具有良好的协同效应，在DA的检测中表现出良好的电催化能力。用Nafion修饰电极后，有效地消除了UA和AA对电极的干扰。NiAl LDH/GLBL修饰电极对DA的氧化具有良好的电化学传感性能，其线性范围宽，实际检测下限为2 nm(S/N=3)。生物传感器由于具有较高的检测效率，已成功地应用于活体人神经细胞受刺激后DA外流的实时跟踪。我们相信，通过将石墨烯直接插入到LDHs材料的层间走廊中，我们的结构集成、有序的LBL异质组装的生物传感平台将为疾病诊断窗口开辟新的途径。(C)2018爱思唯尔B.V.保留所有权利。
This study introduces a new strategy for periodic stacking of positively charged NiAl layered double hydroxides (LDHs) nanosheets with negatively charged monolayers of graphene (G) by systematically optimizing several parameters in a controlled co-feeding fashion and resultant heterostacked NiAl LDH/G LBL nanocomposites have been practically applied in sensitive detection of dopamine released from live cells as early Parkinson's disease (PD) diagnostic tool. PD is the second most chronic neurodegenerative disorder with gradual progressive loss of movement and muscle control causing substantial disability and threatening the life seriously. Unfortunately majority of dopaminergic neurons present in substantia nigra of PD patients are destroyed before it is being clinically diagnosed, so early stages PD diagnosis is essential. Because of direct neighboring of extremely conductive graphene to semiconductive LDHs layers, enhanced intercalation capability of LDHs, and huge surface area with numerous active sites, good synergy effect is harvested in heteroassembled NiAl LDH/G LBL material, which in turn shows admirable electrocatalytic ability in DA detection. The interference induced by UA and AA is effectively eliminated especially after the modifying the electrode with Nafion. The outstanding electrochemical sensing performance of NiAl LDH/G LBL modified electrode has been achieved in terms of broad linear range and lowest real detection limit of 2 nM (S/N = 3) towards DA oxidation. Benefitting from superior efficiency, biosensor has been successfully used for real-time in-vitro tracking of DA efflux from live human nerve cell after being stimulated. We believe that our biosensing platform of structurally integrated well-ordered LBL heteroassembly by inserting graphene directly to the interlayer galleries of LDHs material will open up new avenue in diseases determination window. (c) 2018 Elsevier B.V. All rights reserved.