Psb29, a conserved 22-kD protein, functions in the biogenesis of photosystem II complexes in Synechocystis and Arabidopsis
Psb29, a conserved 22-kD protein, functions in the biogenesis of photosystem II complexes in Synechocystis and Arabidopsis
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DOI：
10.1105/tpc.105.035048
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发表时间：
2005-10-01
期刊：
影响因子：
11.6
通讯作者：
Pakrasi, HB
中科院分区：
文献类型：
--
作者：
Keren, N;Ohkawa, H;Pakrasi, HB
光系统II（PSII）是负责光合放氧的酶，是一种快速翻转的膜蛋白复合物。然而，调节PSII生物发生的因素却定义不清。以前的蛋白质组学分析的PSII制剂从蓝藻集胞藻属PCC 6803检测到一种新的蛋白质，Psb 29（Sll 1414），其同系物中发现的所有蓝藻和维管植物的基因组测序。psb 29的缺失在集胞藻6803中导致在高光强下生长速率较慢，增加的光敏感性，和较低的PSII效率，而不影响PSII核心电子传递活动。拟南芥PSB 29基因中的T-DNA插入系显示出与集胞藻突变体相似的表型。该突变体生长缓慢，叶片呈杂色，PSII活性对光敏感。蓝藻和植物突变体的低温荧光发射光谱显示，增加生长光强度的函数在PSII的非耦合近端天线的比例增加。在植物和蓝藻突变体中观察到的相似表型表明，Psb 29的功能在整个产氧光合生物的进化过程中一直是保守的，并表明Psb 29蛋白在PSII的生物发生中的作用。
Photosystem II (PSII), the enzyme responsible for photosynthetic oxygen evolution, is a rapidly turned over membrane protein complex. However, the factors that regulate biogenesis of PSII are poorly defined. Previous proteomic analysis of the PSII preparations from the cyanobacterium Synechocystis sp PCC 6803 detected a novel protein, Psb29 (Sll1414), homologs of which are found in all cyanobacteria and vascular plants with sequenced genomes. Deletion of psb29 in Synechocystis 6803 results in slower growth rates under high light intensities, increased light sensitivity, and lower PSII efficiency, without affecting the PSII core electron transfer activities. A T-DNA insertion line in the PSB29 gene in Arabidopsis thaliana displays a phenotype similar to that of the Synechocystis mutant. This plant mutant grows slowly and exhibits variegated leaves, and its PSII activity is light sensitive. Low temperature fluorescence emission spectroscopy of both cyanobacterial and plant mutants shows an increase in the proportion of uncoupled proximal antennae in PSII as a function of increasing growth light intensities. The similar phenotypes observed in both plant and cyanobacterial mutants demonstrate that the function of Psb29 has been conserved throughout the evolution of oxygenic photosynthetic organisms and suggest a role for the Psb29 protein in the biogenesis of PSII.