Effects of microwave radiation on anti-infective factors in human milk.
Effects of microwave radiation on anti-infective factors in human milk.
复制标题
微波辐射对母乳中抗感染因子的影响。
作者：
Richard Quan;Christine Yang;Steven Rubinstein;Norman J. Lewiston;P. Sunshine;David K. Stevenson;John A. Kerner
在重症监护托儿所中，使用微波解冻冷冻人乳以更快地获取已成为常见做法。22个新鲜冷冻人乳样品进行了溶菌酶活性，总IgA，特异性分泌IgA大肠杆菌血清型01，04和06。将样品在低功率或高功率设置下通过微波加热30秒，然后再分析。将另外10份人乳样品的ImL等分试样在低（20 ° C至25 ° C）、中（60 ° C至70 ° C）和高（大于或等于98 ° C）设置下微波加热，然后添加至ImL稀释的大肠杆菌悬浮液中的每一个。在37 ℃孵育3 1/2小时后测定大肠杆菌生长。在高温（72摄氏度至98摄氏度）下微波导致所有测试的抗感染因子的活性显著降低。大肠杆菌在大于或等于98摄氏度的生长是对照母乳的18倍。在低温（20 ℃至53 ℃）下微波对总IgA、大肠杆菌血清型01和04的特异性IgA没有显著影响，但显著降低了溶菌酶和大肠杆菌血清型06的特异性IgA。即使在20摄氏度到25摄氏度的温度下，大肠杆菌的生长量也是对照母乳的五倍。微波似乎在高温下是禁忌的，即使在低温下也存在关于其安全性的问题。
In intensive care nurseries it has become common practice to use microwave thawing of frozen human milk for more rapid accessibility. Twenty-two freshly frozen human milk samples were tested for lysozyme activity, total IgA, and specific secretory IgA to Escherichia coli serotypes 01, 04, and 06. The samples were heated by microwave for 30 seconds at a low- or high-power setting and then reanalyzed. One-mL aliquots of 10 additional human milk samples were microwaved at low (20 degrees C to 25 degrees C), medium (60 degrees C to 70 degrees C), and high (greater than or equal to 98 degrees C) setting before the addition to each of 1 mL of diluted E coli suspension. E coli growth was determined after 3 1/2 hours of incubation at 37 degrees C. Microwaving at high temperatures (72 degrees C to 98 degrees C) caused a marked decrease in activity of all the tested antiinfective factors. E coli growth at greater than or equal to 98 degrees C was 18 times that of control human milk. Microwaving at low temperatures (20 degrees C to 53 degrees C) had no significant effect on total IgA, specific IgA to E coli serotypes 01 and 04, but did significantly decrease lysozyme and specific IgA to E coli serotype 06. Even at 20 degrees C to 25 degrees C, E coli growth was five times that of control human milk. Microwaving appears to be contraindicated at high temperatures, and questions regarding its safety exist even at low temperatures.