THE ACT CLUSTER CONTAINS REGULATORY AND ANTIBIOTIC EXPORT GENES, DIRECT TARGETS FOR TRANSLATIONAL CONTROL BY THE BLDA TRANSFER-RNA GENE OF STREPTOMYCES
THE ACT CLUSTER CONTAINS REGULATORY AND ANTIBIOTIC EXPORT GENES, DIRECT TARGETS FOR TRANSLATIONAL CONTROL BY THE BLDA TRANSFER-RNA GENE OF STREPTOMYCES
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DOI：
10.1016/0092-8674(91)90120-n
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发表时间：
1991-08-23
期刊：
影响因子：
64.5
通讯作者：
MALPARTIDA, F
中科院分区：
文献类型：
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作者：
FERNANDEZMORENO, MA;CABALLERO, JL;MALPARTIDA, F
在25 kb的act簇中，actII区两侧是生物合成基因。coelicolor由四个开放阅读框组成，包括生物合成基因的转录激活因子和控制抗生素输出的基因。TTA密码子（在链霉菌中极其罕见）存在于actII-ORF 2（编码推定的跨膜输出蛋白）和actII-ORF 4（转录激活因子基因）中。ORF 4中的TTA变为TTG可以逆转多效性调节基因bldA（编码细胞的tRNA（UUA）Leu）突变引起的放线菌紫素合成的正常中断。我们的结论是，启动放线菌紫素合成通过actII-ORF 4产品，并在生产的最后一步，抗生素出口，是双目标，通过bldA发挥发展控制放线菌紫素生产。
The actII region, flanked by biosynthetic genes in the 25 kb act cluster of S. coelicolor, consists of four open reading frames, including a transcriptional activator for the biosynthetic genes, and genes controlling antibiotic export. A TTA codon (extremely rare in Streptomyces) is present both in actII-ORF2 (encoding a putative transmembrane export protein) and actII-ORF4 (the transcriptional activator gene). Change of the TTA in ORF4 to TTG reverses the normal interruption of actinorhodin synthesis caused by mutation in the pleiotropic regulatory gene bldA (which encodes the cell's tRNA(UUA)Leu). We conclude that initiation of actinorhodin synthesis via the actII-ORF4 product, and the final step in production, antibiotic export, are twin targets via which bldA exerts developmental control of actinorhodin production.