Chemical Nature of a New Antihypertensive Peptide Derived from Jellyfish
Chemical Nature of a New Antihypertensive Peptide Derived from Jellyfish
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DOI：
10.3136/fstr.16.333
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发表时间：
2010-07
影响因子：
0.6
通讯作者：
Yae Morinaga;K. Iwai;H. Tomita;Y. Takaya;Tetsushi Naraoka;H. Matsue
中科院分区：
文献类型：
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作者：
Yae Morinaga;K. Iwai;H. Tomita;Y. Takaya;Tetsushi Naraoka;H. Matsue
采用滚筒干燥法制备了近期日本渔业大流行的野口水母（Stomolophus nomurai）粉末，制备了具有血管紧张素i转换酶（ACE）抑制活性的高浓度（20%）降压肽溶液。用Sep-Pak Vac C18逐级洗脱从多肽混合物中分离出Fr. 3-1、Fr. 3-3和Fr. 3-6三个活性组分，并用高效液相色谱进一步分离两次。测定Fr. 3-1、Fr. 3-3、Fr. 3-6的序列分别为igdepanyl、FGGIDDINQIGQSD、YYAPFE。通过序列分析、MALDI-TOF质谱和核磁共振谱分析，鉴定合成的YYAPFE为活性最强的肽段。此外，在YYAPFE基础上设计合成的YYAPFQ在体外表现出YYAPFE、YYAPF、YYAP、YYA和YY中最高的ACE抑制活性；但其对自发性高血压大鼠收缩压的影响与YYAPFE相似。
A high-concentration (20%) antihypertensive peptide solution with angiotensin I-converting enzyme (ACE) inhibitory activity was prepared from the powder of the jellyfish Stomolophus nomurai, a plague of which had recently hit Japan's fishing industry, using the drum dryer method. Three active fractions, Fr. 3-1, Fr. 3-3, and Fr. 3-6, were isolated from the peptide mixture by step-wise elution by Sep-Pak Vac C18 and further fractionated by high-performance liquid chromatography twice. The sequences of Fr. 3-1, Fr. 3-3, and Fr. 3-6 were determined as IGDEPLANYL, FGGIDDINQIGQSD, and YYAPFE, respectively. The most active peptide was identified with the synthetic peptide YYAPFE by sequence analysis, MALDI-TOF MS, and NMR spectra. Further, YYAPFQ, designed and synthesized on the basis of YYAPFE, showed the highest ACE inhibitory activity in vitro among YYAPFE, YYAPF, YYAP, YYA, and YY; however, its effect on systolic blood pressure showed a similar tendency to that of YYAPFE in spontaneously hypertensive rats.