MUTATION ASSAY AT THE THYMIDINE KINASE LOCUS IN DIPLOID HUMAN-LYMPHOBLASTS
MUTATION ASSAY AT THE THYMIDINE KINASE LOCUS IN DIPLOID HUMAN-LYMPHOBLASTS
复制标题
DOI：
10.1016/0027-5107(82)90308-6
复制
发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
THILLY, WG
中科院分区：
文献类型：
--
作者：
LIBER, HL;THILLY, WG
从二倍体人淋巴母细胞系中分离出胸苷激酶杂合子，并在微滴皿中高效形成菌落。这种被称为TK6的细胞系可以通过多种诱变剂从TK+/-状态突变为TK-/-状态，包括甲磺酸乙酯、甲磺酸丁酯、亚硝基甲基脲、紫外线、icr - 191,4 -硝基喹啉氧化物、氟脱氧尿嘧啶、苯并[a]芘和黄曲霉毒素B1。实验需要证明这一新的线在定量分析人类细胞突变的适用性报告。二甲亚砜或phorl -12-肉豆蔻酸-13-醋酸酯均不能诱导着丝粒与tk位点之间的有丝分裂重组。
A thymidine kinase heterozygote was isolated from a diploid human lymphoblast line which forms colonies with high efficiency in microtiter dishes. This cell line, called TK6, can be mutated from a TK+/- to TK-/- state by diverse mutagens, including ethyl methanesulfonate, butyl methanesulfonate, nitrosomethylurea, UV light, ICR-191, 4-nitroquinoline oxide, fluorodeoxyuridine, benzo[a]pyrene and aflatoxin B1. The experiments required to demonstrate the applicability of this new line in quantitative assays of mutation in human cells are reported. Mitotic recombination between the centromere and the tk locus could not be induced by either dimethylsulfoxide or phorbol-12-myristate-13-acetate.