Stereospecific Isotopic Labeling of Methyl Groups for NMR Spectroscopic Studies of High-Molecular-Weight Proteins
Stereospecific Isotopic Labeling of Methyl Groups for NMR Spectroscopic Studies of High-Molecular-Weight Proteins
复制标题
DOI：
10.1002/anie.200905660
复制
发表时间：
2010-01-01
影响因子：
16.6
通讯作者：
Boisbouvier, Jerome
中科院分区：
文献类型：
--
作者：
Gans, Pierre;Hamelin, Olivier;Boisbouvier, Jerome
有时候少就是多：[13 C1 H3]甲基同位素异构体可以通过使用乙酰乳酸盐前体在全氘代背景下生物合成地特异性掺入到大蛋白质组装体中亮氨酸和缬氨酸残基的pro-S甲基中。这种立体特异性标记策略大大增强了大蛋白组装体的NMR谱。
Sometimes less is more: [13C1H3]methyl isotopomers can be biosynthetically incorporated specifically into the pro-S methyl groups of leucine and valine residues in large protein assemblies within a perdeuterated background by using an acetolactate precursor. This stereospecific labeling strategy considerably enhances NMR spectra for large protein assemblies.