Interaction of pro-apoptotic protein HGTD-P with heat shock protein 90 is required for induction of mitochondrial apoptotic cascades
Interaction of pro-apoptotic protein HGTD-P with heat shock protein 90 is required for induction of mitochondrial apoptotic cascades
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DOI：
10.1016/j.febslet.2006.05.001
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发表时间：
2006-05-29
期刊：
影响因子：
3.5
通讯作者：
Park, Jae-Hoon
中科院分区：
文献类型：
--
作者：
Kim, Jee-Youn;Kim, Su-Mi;Park, Jae-Hoon
HGTD-P是一种缺氧反应性促凋亡蛋白，可将缺氧信号直接传递至线粒体。当过表达时，HGTD-P通过典型的线粒体凋亡级联诱导细胞死亡。然而，HGTD-P与细胞死亡调节蛋白相关的转录后修饰和信号传导网络仍知之甚少。我们进行酵母双杂交筛选，以确定参与HGTD-P介导的细胞死亡途径的分子。在这项研究中，我们表明，热休克蛋白90物理相互作用与HGTD-P和Hsp 90活性的抑制低浓度格尔德霉素减少HGTD-P诱导的线粒体灾难通过抑制线粒体易位的HGTD-P。（c）2006年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
HGTD-P is a hypoxia-responsive pro-apoptotic protein that transmits hypoxic signals directly to mitochondria. When overexpressed, HGTD-P induces cell death via typical mitochondrial apoptotic cascades. However, much is unknown about post-transcriptional modification and signaling networks of HGTD-P in association with cell death-regulating proteins. We performed yeast two-hybrid screening to identify the molecules involved in HGTD-P-mediated cell death pathways. In this study, we show that heat shock protein 90 physically interacts with HGTD-P and that suppression of Hsp90 activity by low concentrations of geldanamycin reduced HGTD-P-induced mitochondrial catastrophe through inhibition of mitochondrial translocation of HGTD-P. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.