Examination of Ion Channel Protein Orientation in Supported Lipid Bilayers
Examination of Ion Channel Protein Orientation in Supported Lipid Bilayers
复制标题
支撑脂质双层中离子通道蛋白方向的检查
DOI：
10.1143/apex.4.107001
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发表时间：
2011
影响因子：
2.3
通讯作者：
K. Torimitsu
中科院分区：
文献类型：
--
作者：
Youichi Shinozaki;K. Sumitomo;Aya Tanaka;N. Kasai;K. Torimitsu
我们研究了调节脂质双层中离子通道型膜蛋白重组方向的技术。自由离子通道蛋白将其长轴平行于基底排列。相反，免疫化学和原子力显微镜图像显示，离子通道在向上的支撑脂质双层中重建，其长轴垂直于基底。我们的数据表明，通过破坏小单层蛋白脂质体将离子通道重建为平面脂质双层是一种在膜中向上排列离子通道并获得具有受控功能的离子通道的有前途的方法。
We investigated techniques for regulating the orientation of ion channel-type membrane proteins reconstituted in lipid bilayers. Free ion channel proteins aligned their long axis parallel to the substrate. In contrast, immunochemical and atomic force microscopy images revealed that ion channels reconstituted in supported lipid bilayers oriented upward, with their long axis perpendicular to the substrate. Our data demonstrates that the reconstitution of ion channels into planar lipid bilayers by rupturing small unilamellar proteoliposomes is a promising way of aligning ion channels upward in a membrane and of obtaining ion channels with controlled functions.
影响因子：
64.8
作者：
Miyazawa, A;Fujiyoshi, Y;Unwin, N
通讯作者：
Unwin, N