ZnO nanoparticles affect nutrient transport in an in vitro model of the small intestine
ZnO nanoparticles affect nutrient transport in an in vitro model of the small intestine
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DOI：
10.1016/j.fct.2018.11.048
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发表时间：
2019-02-01
影响因子：
4.3
通讯作者：
Mahler, Gretchen J.
中科院分区：
文献类型：
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作者：
Moreno-Olivas, Fabiola;Tako, Elad;Mahler, Gretchen J.
纳米氧化锌存在于食品包装中，使消费者面临摄入的风险。关于食品包装中存在的纳米氧化锌的数量以及摄入纳米氧化锌对肠道功能的影响的信息很少。为了评估通常包装有氧化锌纳米粒的食品中与生理相关的氧化锌纳米粒暴露，采用电感耦合等离子体质谱(ICP-MS)对食品样品进行了分析。采用体外小肠模型，研究了氧化锌-NP暴露对小鼠小肠的影响。细胞暴露在培养液中的原始NP中，并接受NP的体外消化过程，以更好地反映NP在人类胃肠道(GI)中经历的转化。结果表明，生理剂量的氧化锌纳米粒可导致葡萄糖转运显著减少，这与基侧葡萄糖转运蛋白GLUT2基因表达的变化一致。也有证据表明，氧化锌纳米粒影响肠道细胞的微绒毛，因此减少了可用于吸收营养的表面积。这些结果表明，在人体小肠的体外模型中，摄入氧化锌NP可以改变营养物质的吸收。
Nano-sized zinc oxide (ZnO) is present in food packaging, putting consumers at risk of ingestion. There is little information on the amount of ZnO nanoparticles (NP) present in food packaging and the effects of ZnO NP ingestion on intestinal function. To estimate physiologically relevant ZnO NP exposures from food that are commonly packaged with ZnO NP, food samples were analyzed with inductively coupled plasma mass spectrometry (ICP-MS). An in vitro model of the small intestine was used to investigate the effects of ZnO NP exposure. Cells were exposed to pristine NP in culture medium and to NP subjected to an in vitro digestion process to better reflect the transformation that the NP undergo in the human gastrointestinal (GI) tract. The findings show that a physiologically relevant dose of ZnO NP can cause a significant decrease in glucose transport, which is consistent with gene expression changes for the basolateral glucose transporter GLUT2. There is also evidence that the ZnO NP affect the microvilli of the intestinal cells, therefore reducing the amount of surface area available to absorb nutrients. These results suggest that the ingestion of ZnO NP can alter nutrient absorption in an in vitro model of the human small intestine.