Tailoring Cell Behavior on Polymers by the Incorporation of Titanium Doped Phosphate Glass Filler
Tailoring Cell Behavior on Polymers by the Incorporation of Titanium Doped Phosphate Glass Filler
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DOI：
10.1002/adem.200980083
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发表时间：
2010-07-01
影响因子：
3.6
通讯作者：
Knowles, Jonathan C.
中科院分区：
文献类型：
--
作者：
Chrzanowski, Wojciech;Abou Neel, Ensanya A.;Knowles, Jonathan C.
了解组织对材料的反应，以实现调制和引导组织再生是生物材料科学的主要挑战之一。如今，聚合物、玻璃和金属作为生物材料占主导地位。通常，这些材料的天然性质是不够的，并且需要将它们联合收割机组合，以便根据应用改变和调整它们的性质。本研究的主要目的是使用磷酸盐玻璃作为填料（钛掺杂磷酸盐玻璃）来改善细胞对聚合物（PLDL）的反应。作为对照，使用β-磷酸三钙（TCP）填料。使用各种浓度的填料（10-40体积%）。润湿行为，zeta电位，机械和热性能，以及人体细胞对材料的反应进行了评价。结果表明，随着玻璃填料含量的增加，润湿性得到改善，zeta电位下降，材料的刚度增加。重要的是，细胞培养实验显示，玻璃含量高达20体积%的样品上的细胞更发达且分布良好。细胞对玻璃填充的样品的反应比TCP填充的积极得多。然而，骨钙素和骨桥蛋白的表达，表明形成的矿化结构的蛋白质是阳性的所有样品，包括纯PLDL。得出的结论是，由于改善的润湿行为，较低的zeta电位，和特定的化学玻璃填料，它是可能的，以改变细胞的反应，提高生物活性的聚合物，并改变机械性能。
Understanding tissue response to materials, to enable modulation and guided tissue regeneration is one of the main challenges in biomaterials science. Nowadays polymers, glasses, and metals dominate as biomaterials. Often native properties of those materials are not sufficient and there is a need to combine them, so as to modify and adjust their properties to the application. The primary aim of this study was to improve cell response to polymer (PLDL) using phosphate glass as filler (titanium doped phosphate glass). As a control beta-tricalcium phosphate (TCP) filler was used. Various concentrations of the filler were used (10-40 vol%). Wetting behavior, zeta-potentials, mechanical and thermal properties, and human cells response to the materials were evaluated. Results showed that with increase in glass filler loading wettability improved, zeta-potentials dropped, and increase in stiffness of materials was observed. Importantly cell culture experiments showed more developed and well spread cells on the samples with glass content up to 20 vol%. Cells responded much more positively to the glass filled samples than to TCP filled. However, expression of osteocalcin and osteopontin, proteins that indicate formation of the mineralized structures was positive for all the samples including pure PLDL. It was concluded that due to improved wetting behavior, lower zeta-potentials, and specific chemistry of the glass filler it was possible to alter cells response, improve bioactivity of the polymer, and vary mechanical properties.