Cell-type specific expression of a transfected immunoglobulin gene
Cell-type specific expression of a transfected immunoglobulin gene
复制标题
转染的免疫球蛋白基因的细胞类型特异性表达
作者：
J. Stafford;C. Queen
将克隆基因导入真核细胞已成为基因表达研究的主要技术。许多实验已经证明了克隆基因在转染到异源细胞系统中后的转录，在异源细胞系统中基因通常是不活跃的1 -4。最近，一些研究者已经在转染到相应特化类型的细胞中后获得了特化基因的表达，特别是红白血病细胞中的β-珠蛋白基因5和骨髓瘤细胞中的免疫球蛋白基因6，7。这些结果允许通过比较转染到同源和异源细胞中的基因的转录来研究发育期间的基因表达。我们已经证明，从小鼠骨髓瘤中克隆的重排κ免疫球蛋白基因在重新引入小鼠骨髓瘤细胞时以高水平瞬时转录8。我们在这里显示，在一个内部控制的方式，相同的免疫球蛋白基因是不可检测的转录时，转染到小鼠3 T3或L细胞。
The introduction of cloned genes into eukaryotic cells has become a major technique in the study of gene expression. Many experiments have demonstrated transcription of cloned genes after transfection into heterologous cell systems—cells in which the genes are not normally active1–4. More recently, several investigators have obtained expression of specialized genes after transfection into cells of the corresponding specialized type, notably the β-globin gene in erythroleukaemic cells5 and immunoglobulin genes in myeloma cells6,7. These results allow the study of gene expression during development by comparing transcription of a gene transfected into homologous and heterologous cells. We have shown that a rearranged κ immunoglobulin gene, cloned from a mouse myeloma, is transcribed transiently at a high level when reintroduced into mouse myeloma cells8. We show here, in an internally controlled manner, that the same immunoglobulin gene is not detectably transcribed when transfected into mouse 3T3 or L cells.
DOI：
10.1073/pnas.80.3.825
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
OI, VT;MORRISON, SL;BERG, P
通讯作者：
BERG, P