A Label-Free Photoluminescence Genosensor Using Nanostructured Magnesium Oxide for Cholera Detection.
A Label-Free Photoluminescence Genosensor Using Nanostructured Magnesium Oxide for Cholera Detection.
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DOI：
10.1038/srep17384
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发表时间：
2015-11-27
影响因子：
4.6
通讯作者：
Malhotra BD
中科院分区：
文献类型：
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作者：
Patel MK;Ali MA;Krishnan S;Agrawal VV;Al Kheraif AA;Fouad H;Ansari ZA;Ansari SG;Malhotra BD
基于纳米材料的光致发光（PL）诊断设备可快速、高灵敏度地检测农药、DNA和有毒物质。在这里，我们报告了一种无标记的荧光基因传感器的敏感检测霍乱弧菌，是基于DNA杂交策略，利用纳米结构的氧化镁（nMgO;大小>30 nm）颗粒。通过透射电子显微镜（TEM）研究确定了合成的nMgO的形貌和尺寸。探针DNA（pDNA）与nMgO的共轭和其特征在于通过X射线光电子和傅里叶变换红外光谱技术。使用pDNA-nMgO复合物对从霍乱弧菌临床样品中分离的靶互补基因组DNA（cDNA）进行DNA杂交研究，并通过测量PL强度的变化来完成cDNA的检测。在700 nm处测量的PL峰强度（红色发射）随着cDNA浓度的增加而增加。该传感器的线性范围为100 ~ 500 ng/μL，灵敏度为1.306 emi/ng，检出限为3.133 ng/μL，线性回归系数（R2）为0.987。这些结果表明，这种超灵敏的PL基因传感器具有潜在的应用于霍乱的临床诊断。
Nanomaterial-based photoluminescence (PL) diagnostic devices offer fast and highly sensitive detection of pesticides, DNA, and toxic agents. Here we report a label-free PL genosensor for sensitive detection of Vibrio cholerae that is based on a DNA hybridization strategy utilizing nanostructured magnesium oxide (nMgO; size >30 nm) particles. The morphology and size of the synthesized nMgO were determined by transmission electron microscopic (TEM) studies. The probe DNA (pDNA) was conjugated with nMgO and characterized by X-ray photoelectron and Fourier transform infrared spectroscopic techniques. The target complementary genomic DNA (cDNA) isolated from clinical samples of V. cholerae was subjected to DNA hybridization studies using the pDNA-nMgO complex and detection of the cDNA was accomplished by measuring changes in PL intensity. The PL peak intensity measured at 700 nm (red emission) increases with the increase in cDNA concentration. A linear range of response in the developed PL genosensor was observed from 100 to 500 ng/μL with a sensitivity of 1.306 emi/ng, detection limit of 3.133 ng/μL and a regression coefficient (R2) of 0.987. These results show that this ultrasensitive PL genosensor has the potential for applications in the clinical diagnosis of cholera.