Thermoplastic microfluidic device for on-chip purification of nucleic acids for disposable diagnostics
Thermoplastic microfluidic device for on-chip purification of nucleic acids for disposable diagnostics
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DOI：
10.1021/ac051449j
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发表时间：
2006-02-01
影响因子：
7.4
通讯作者：
Klapperich, CM
中科院分区：
文献类型：
--
作者：
Bhattacharyya, A;Klapperich, CM
一种用于基于固相萃取（SPE）的核酸分离的聚合物微流体装置被证明。塑料芯片可以用作用于不同生物和诊断应用的一次性样品制备系统。芯片是由一个环状聚烯烃热压与主模具。固相由用二氧化硅颗粒浸渍的多孔整体聚合物柱组成。由于核酸与整料中的二氧化硅颗粒的结合，实现了提取。通过甲基丙烯酸酯和二甲基丙烯酸酯单体的混合物、UV敏感的自由基引发剂和致孔溶剂的原位光引发聚合，在装置的通道内形成固相。通道表面通过光接枝进行预处理，以将整料共价连接到通道壁。通过该方法制备的固相允许成功提取和洗脱聚合物微芯片中的核酸。
A polymeric microfluidic device for solid-phase extraction (SPE)-based isolation of nucleic acids is demonstrated. The plastic chip can function as a disposable sample preparation system for different biological and diagnostic applications. The chip was fabricated in a cyclic polyolefin by hot-embossing with a master mold. The solid phase consisted of a porous monolithic polymer column impregnated with silica particles. The extraction was achieved due to the binding of nucleic acids to the silica particles in the monolith. The solid phase was formed within the channels of the device by in situ photoinitiated polymerization of a mixture of methacrylate and dimethacrylate monomers, UV-sensitive free-radical initiator, and porogenic solvents. The channel surfaces were pretreated via photografting to covalently attach the monolith to the channel walls. The solid phase prepared by this method allowed for successful extraction and elution of nucleic acids in the polymeric microchip.