EVIDENCE FOR AN ESSENTIAL NON-WATSON-CRICK INTERACTION BETWEEN THE 1ST AND LAST NUCLEOTIDES OF A NUCLEAR PREMESSENGER RNA INTRON
EVIDENCE FOR AN ESSENTIAL NON-WATSON-CRICK INTERACTION BETWEEN THE 1ST AND LAST NUCLEOTIDES OF A NUCLEAR PREMESSENGER RNA INTRON
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DOI：
10.1038/361660a0
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发表时间：
1993-02-18
期刊：
影响因子：
64.8
通讯作者：
SILICIANO, PG
中科院分区：
文献类型：
--
作者：
PARKER, R;SILICIANO, PG
核前信使RNA剪接需要5个小的核(Sn)RNA，U1，U2，U4，U5和U6以及50多个蛋白质1，2。核前mRNA剪接和第二组自剪接的机制相似表明，核前mRNA剪接的许多中央过程是基于RNA-RNA相互作用的3，4。要了解前mRNA剪接的机制，必须确定剪接过程中SNRNA和前mRNA之间的相互作用及其时间关系。已经证实了几种SnRNA-SnRNA5-9和SnRNA-内含子9-12的相互作用，但在3‘切割和外显子连接过程中，是否有可能识别3’剪接位点AG二核苷酸的基于RNA的相互作用尚不清楚。我们报道了酵母肌动蛋白内含子5‘和3’剪接位点突变之间的相互抑制，并提出3‘剪接位点至少部分地通过5’和3‘剪接位点鸟苷之间的非Watson-Crick相互作用来定位于3’切割和外显子连接。
NUCLEAR pre-messenger RNA splicing requires the action of five small nuclear (sn) RNAs, U1, U2, U4, U5 and U6, and more than 50 proteins1,2. The mechanistic similarity of nuclear pre-mRNA splicing and group II self-splicing suggests that many of the central processes of nuclear pre-mRNA splicing are based on RNA-RNA interactions3,4. To understand the mechanism of pre-mRNA splicing, the interactions, and their temporal relationships, that occur between the snRNAs and the pre-mRNA during splicing must be identified. Several snRNA-snRNA5-9 and snRNA-intron9-12 interactions have been demonstrated but the putative RNA-based interactions that recognize the AG dinucleotide at the 3' splice site during 3' cleavage and exon ligation are unknown. We report here the reciprocal suppression between 5' and 3' splice site mutations in the yeast actin intron, and propose that the 3' splice site is positioned for 3' cleavage and exon ligation, at least in part, through a non-Watson-Crick interaction between the guanosines at the 5' and 3' splice sites.