THE EFFECT OF OSTEOGENIN (A BONE MORPHOGENETIC PROTEIN) ON THE FORMATION OF BONE IN ORTHOTOPIC SEGMENTAL DEFECTS IN RATS
THE EFFECT OF OSTEOGENIN (A BONE MORPHOGENETIC PROTEIN) ON THE FORMATION OF BONE IN ORTHOTOPIC SEGMENTAL DEFECTS IN RATS
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DOI：
10.2106/00004623-199411000-00011
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发表时间：
1994-11-01
影响因子：
5.3
通讯作者：
REDDI, AH
中科院分区：
文献类型：
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作者：
STEVENSON, S;CUNNINGHAM, N;REDDI, AH
我们研究了部分纯化的天然成骨蛋白（一种骨形态发生蛋白）对大鼠骨形成的影响。在36只成年雄性Fischer大鼠的双侧股骨干中部造成8 mm宽的骨骨膜节段性缺损，并用聚乙酰钢板和螺纹克氏针固定。一个缺损用含有60%羟基磷灰石和40%磷酸三钙陶瓷（孔径，250至400微米）的圆柱体填充，该圆柱体含有100微克部分纯化的牛成骨素，对侧缺损用不含成骨素的羟基磷灰石-磷酸三钙陶瓷圆柱体填充。对18只动物（术后1个月、2个月和4个月各6只动物）进行了组织学和组织形态学研究。另外18只动物的植入物（术后1、2和4个月各6只动物）进行生物力学测试。组织形态测定显示骨的总面积，植入物外的骨面积，和植入物孔隙内的骨量都显著增加，（p小于或等于0.05）在大多数时间段内，具有含成骨蛋白的植入物的股骨比具有不含成骨蛋白的植入物的股骨更大。骨生成素的存在下，没有显着的影响，在本study.CLINICAL RELEVANCE测量的生物力学参数：骨丢失由于创伤，切除肿瘤，或松动和下沉的关节假体是一个主要的临床问题。虽然骨具有显著的再生潜力，但在某些骨科情况下，可预测的自然修复过程的增强将是有用的。特别有吸引力的是骨修复的增强，而不需要松质自体骨移植，这需要第二次手术。在这项研究中，含骨生成素的陶瓷圆柱体显著（p小于或等于0.005）增强了大段缺损内和周围的骨形成。这种增强的修复发生在没有添加松质自体骨移植物或骨髓细胞的情况下，并表明成骨素可能在骨骼缺损的修复中是有用的，并且这种陶瓷结构可能是成骨素的有用载体。
We studied the effects of partially purified, natural osteogenin, a bone morphogenetic protein, on the formation of bone in rats. An osteoperiosteal segmental defect, eight millimeters wide, in the middle of the femoral diaphysis was created bilaterally in thirty-six adult male Fischer rats and stabilized with a polyacetyl plate and threaded Kirschner wires. One defect was filled with a cylinder of 60 per cent hydroxyapatite and 40 per cent tricalcium phosphate ceramic (pore diameter, 250 to 400 micrometers) containing 100 micrograms of partially purified bovine osteogenin, and the contralateral defect was filled with a hydroxyapatite-tricalcium ceramic cylinder without osteogenin. Eighteen animals (six animals each at one, two, and four months after the operation) were studied histologically and histomorphometrically. The implants from eighteen additional animals (six animals each at one, two, and four months after the operation) were subjected to biomechanical testing.Histomorphometry revealed that the total area of bone, the area of bone outside of the implant, and the amount of bone within the pores of the implant were all significantly (p less than or equal to 0.05) greater in the femora that had an implant with osteogenin than in those that had an implant without osteogenin at most time-periods. The presence of osteogenin had no significant effect on the biomechanical parameters measured in this study.CLINICAL RELEVANCE: Bone loss due to trauma, excision of a tumor, or loosening and subsidence of a joint prosthesis is a major clinical problem. Although bone has a remarkable potential for regeneration, predictable augmentation of the natural repair process would be useful in certain orthopaedic situations. Particularly attractive would be the augmentation of bone repair without the need for a cancellous autogenous bone graft, which necessitates a second operative procedure. In this study, an osteogenin-containing ceramic cylinder significantly (p less than or equal to 0.005) enhanced the formation of bone in and around a large segmental defect. This enhanced repair occurred without the addition of cancellous autogenous bone graft or marrow cells and suggests that osteogenin may be useful in the repair of skeletal defects and that this ceramic configuration may be a useful carrier of osteogenin.