Cosmid DNA packaging in vivo.
Cosmid DNA packaging in vivo.
复制标题
粘粒 DNA 体内包装。
DOI：
10.1016/0378-1119(82)90064-6
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发表时间：
1982
期刊：
影响因子：
3.5
通讯作者：
Frackman,S
中科院分区：
文献类型：
--
作者：
Feiss,M;Siegele,DA;Rudolph,CF;Frackman,S
本文描述了粘粒DNA在λ噬菌体生长过程中包装入噬菌体颗粒的过程。有证据支持其他人的工作，即粘粒转导载体含有粘粒DNA的线性多聚体，其中粘粒拷贝数是形成可包装DNA长度所需的数量（大于λ DNA长度的0.77）。随着形成可包装DNA长度所需的粘粒拷贝数的增加，粘粒转导DNA的产量急剧下降。产生包装底物的粘粒DNA复制与λ滚环复制一样依赖于λ基因产物。
The packaging of cosmid DNA into phage particles during phage λ growth is described. Evidence is presented supporting the work of others that cosmid transducing phages contain linear multimers of cosmid DNA in which the number of cosmid copies is that required to make a packagable DNA length (greater than 0.77 of the λ DNA length). The yield of cosmid transducing phages declines sharply as the number of cosmid copies required to make a packagable DNA length increases. The cosmid DNA replication that produces the packaging substrate shares with lambda rolling-circle replication a dependence on the λgarngene product.